The Acute Effect of Spices on Vascular Health

A 3-period randomized controlled crossover study will be conducted to investigate the
postprandial effects of a high fat meal with spices on endothelial function,
lipids/lipoproteins, immune function, plasma antioxidants and markers of oxidative stress.
Metabolomic profiling will also be conducted. In random order participants will consume
either a high fat meal (1000kcal, 45g fat) or a high fat meal containing 2g of spices or a
high fat meal containing 6g of spices with a 3 day washout period between each treatment. The
following spices will be incorporated into the meal black pepper, basil, bay leaf, cinnamon,
coriander, cumin, ginger, oregano, parsley, rosemary, red pepper, turmeric and thyme.

Endothelial function will be measured by flow mediated dilation of the brachial artery in the
fasting state and 2 and 4 hours after the meal. Participants will also provide a fasting
blood sample and samples will also be taken at 1, 2, 3 and 4 hours after the meal.

Inclusion Criteria:

- male aged 40-65 years

- BMI 25-35kg/m2

- nonsmoking

- waist circumference =/> 94cm and at least one other CVD risk factor (elevated LDL-C (>
130 mg/dL), CRP (> 1 mg/L), elevated Triglycerides (≥ 150 mg/dL), reduced
HDL-cholesterol (< 40 mg/dL), elevated blood pressure (systolic BP ≥ 130 or diastolic
BP ≥ 85 mm Hg), elevated fasting glucose (≥ 100 mg/dL))

- low herb/spice consumers (consumption <1/day)

Exclusion Criteria:

- Chronic disease risk factors that are diagnostic of diabetes (fasting glucose > 126
mg/dL) or hypertension (SBP >160 mm Hg or DBP > 100 mm Hg).

- Prescription of anti-hypertensive or glucose lowering drugs.

- Established CVD, stroke, diabetes, liver, kidney or autoimmune disease

- Use of cholesterol/lipid-lowering medication or supplements (psyllium, fish oil, soy
lecithin, and phytoestrogens) and botanicals

- weight loss of ≥10% of body weight within the 6 months prior to enrolling in the study

- vegetarianism