Metabolic Flexibility as a Biomarker of Adaptation to Diet and Exercise Challenges

Inclusion Criteria:

- Males and Females

- Age 22 to 35 years at beginning of study

Exclusion Criteria:

- Women who have given birth during the previous 12 months

- Pregnant women or women who plan to become pregnant or become pregnant during the
study

- Lactating women

- History or presence of diabetes, kidney disease, liver disease, certain cancers,
gastrointestinal, pancreatic, other metabolic diseases, or malabsorption syndromes.

- History of bariatric or certain other surgeries related to weight control

- Smokers or other tobacco users (for at least 6 months prior to the start of the
study)

- History of eating disorders or other dietary patterns which are not consistent with
the dietary intervention (e.g., vegetarians, very low fat diets, high protein diets)

- Volunteers who have lost 10% of body weight within the last 12 months or who plan to
initiate a weight loss program during the next 10 months

- Unable or unwilling to give informed consent or communicate with study staff

- Self-report of alcohol or substance abuse within the past 12 months and/or current
acute treatment or rehabilitation program for these problems (long-term participation
in Alcoholics Anonymous is not an exclusion)

- Other medical, psychiatric, or behavioral factors that in the judgment of the
Principal Investigator may interfere with study participation or the ability to
follow the intervention protocol

- Inclusion and exclusion criteria have been established to recruit a population of
individuals across a wide age-range that has typical digestive process