Neoadjuvant Combination Biotherapy With Ipilimumab and Nivolumab or Nivolumab Alone

The study has 2 study arms:

- Nivolumab alone (Arm A)

- Induction phase: nivolumab 3 mg/kg IV infusion every 2 weeks x3 doses. Followed by
Definitive Surgery.

- Maintenance phase: nivolumab 3 mg/kg IV infusion every 3 weeks for up to one year
from study treatment initiation.

- Ipilimumab + nivolumab (Arm B)

- Induction phase: Ipilimumab 3 mg/kg IV + Nivolumab 1 mg/kg IV every 3 weeks x2.
Followed by Definitive Surgery.

- Maintenance phase: nivolumab 3 mg/kg IV infusion every 2 weeks for up to one year
from study treatment initiation.

Definitive Surgery consists of complete lymph node dissection/ lymphatic, cutaneous,
subcutaneous or other distant disease resection (week 6-8+).

Inclusion Criteria:

- Men and women at least 18 years of age

- Willing and able to give written informed consent

- Performance status Eastern Cooperative Oncology Group (ECOG) zero or 1

- Histologic diagnosis of melanoma belonging to the following AJCC Tumor Node and
Metastasis (TNM) stages: Tx or T1-4 and N1b or N2 or N2c or N3 and/or M0 or M1 (if
considered surgically operable)

- Patients are eligible for this trial either at presentation for primary melanoma with
concurrent regional nodal and/or in-transit metastasis and/or distant metastasis, or
at the time of clinically detected nodal and/or in-transit recurrence and/or distant
metastasis and may belong to any of the following groups: Primary melanoma with
clinically apparent (overt) regional lymph node metastases.

- Clinically detected recurrence of melanoma at the proximal regional lymph node(s)
basin.

- Clinically detected primary melanoma involving multiple regional nodal groups.

- Clinically detected site of nodal metastatic melanoma arising from an unknown
primary.

- Patients with intransit or satellite metastases with or without lymph node
involvement are allowed if they are considered surgically resectable at baseline.

- Patients with distant metastases with or without intransit or lymph node involvement
are allowed if they are considered potentially surgically resectable at baseline.
NOTE: All patients must be determined to be surgically resectable at baseline to be
eligible for this neoadjuvant study.

- Have measurable disease based on RECIST 1.1.

- Have provided tumor tissue from a newly obtained core, punch, incisional or
excisional tumor biopsy. Patients must undergo biopsy (core, punch) or open
incisional/excisional biopsy (if done as part of a clinically indicated baseline
diagnostic procedure) within 4 weeks of registration on the study.

- Patients must have been evaluated by standard-of-care full body imaging studies (CT,
PET/CT or MRI) as part of the initial clinical work-up at baseline (no more than 4
weeks prior to study enrollment) and after completion of induction
nivolumab-ipilimumab or nivolumab alone (at 6-8 weeks after the first dose of
induction and prior to the definitive surgery procedure).

- Required values for initial laboratory tests:

- White blood cell (WBC) 3000/uL

- Absolute neutrophil count (ANC) 1500/uL

- Platelets 100 x 103/uL

- Hemoglobin 10 g/dL

- Aspartate transaminase/alanine transaminase (AST/ALT) 2.5 x unique learner
number (ULN)

- Bilirubin 1.5 ULN, (except patients with Gilbert's Syndrome, who must have a
total bilirubin less than 3.0 mg/dL)

- Serum creatinine ≤ 1.5 x ULN or creatinine clearance (CrCl) ≥ 40 mL/min (if
using the Cockcroft-Gault formula below):

Female CrCl = (140 - age in years) x weight in kg x 0.85 72 x serum creatinine in mg/dL
Male CrCl = (140 - age in years) x weight in kg x 1.00 72 x serum creatinine in mg/dL

• No active or chronic infection with HIV, Hepatitis B, or Hepatitis C

Exclusion Criteria:

- Subject will be excluded from participating in the trial if they meet any of the
following criteria:

- Patients are excluded if they have a history of central nervous system
(CNS)metastases.

- Patients who have had a history of acute diverticulitis, intra-abdominal abscess, GI
obstruction and abdominal carcinomatosis which are known risk factors for bowel
perforation.

- Any other malignancy from which the patient has been disease-free for less than 3
years, with the exception of adequately treated and cured basal or squamous cell skin
cancer, superficial bladder cancer or carcinoma in situ of the cervix.

- Patients will be excluded if they have an active, known or suspected autoimmune
disease. Autoimmune disease: Patients with a history of inflammatory bowel disease
are excluded from this study, as are patients with a history of symptomatic
autoimmune disease (e.g., rheumatoid arthritis, systemic progressive sclerosis
(scleroderma), systemic lupus erythematosus, autoimmune vasculitis e.g., Wegener's
Granulomatosis); motor neuropathy considered of autoimmune origin (e.g.
Guillain-Barre Syndrome).

- Subjects are permitted to enroll if they have vitiligo, type I diabetes mellitus,
residual hypothyroidism due to autoimmune condition only requiring hormone
replacement, psoriasis not requiring systemic treatment, or conditions not expected
to recur in the absence of an external trigger.

- Has evidence of interstitial lung disease or active, non-infectious pneumonitis.

- Patients should be excluded if they have a condition requiring systemic treatment
with either corticosteroids (> 10 mg daily prednisone equivalents) or other
immunosuppressive medications within 14 days of study drug administration. Inhaled or
topical steroids and adrenal replacement doses > 10 mg daily prednisone equivalents
are permitted in the absence of active autoimmune disease.

- As there is potential for hepatic toxicity with nivolumab or nivolumab/ipilimumab
combinations, drugs with a predisposition to hepatoxicity should be used with caution
in patients treated with nivolumab-containing regimen.

- Has an active infection requiring systemic therapy.

- Has received a live vaccine within 30 days prior to the first dose of trial
treatment.

- Any underlying medical or psychiatric condition, which in the opinion of the
Investigator/Sub-Investigator will make the administration of ipilimumab or nivolumab
hazardous or obscure the interpretation of adverse events (AEs), such as a condition
associated with frequent diarrhea.

- Patients with underlying heart conditions who are deemed ineligible for surgery by
cardiology consult.

- A history of prior treatment with ipilimumab, nivolumab or other cytotoxic
T-lymphocyte-associated protein (CTLA-4), progressive disease (PD1) or PD-L1
inhibitor.

- Prior treatment with interferon alfa is allowed.

- A history of prior radiotherapy, chemotherapy, including infusion or perfusion
therapy for current disease or any immunotherapy including tumor vaccines,
interferon-alfa, interleukins, levamisole or other biologic response modifiers within
the past 4 weeks.

- Concomitant therapy with any of the following: IL 2 or other non-study immunotherapy
regimens; cytotoxic chemotherapy; immunosuppressive agents; other investigation
therapies; or chronic use of systemic corticosteroids; unless discontinued ≥ 4 weeks.
A history of occasional use of steroid inhalers is allowed.

- Women of childbearing potential (WOCBP), defined above in Section 3.3, who are
unwilling or unable to use an acceptable method of contraception to avoid pregnancy
for their entire study period and for at least 23 weeks after cessation of study
drug, or have a positive pregnancy test at baseline, or are pregnant or
breastfeeding.

- Melanoma of Uveal Origin