Neoadjuvant Pembrolizumab

The presumed mechanism of action for pembrolizumab is the removal of T lymphocyte inhibition
by masking the PD-1 receptor. Our hypothesis is that the masking of the PD-1 receptor by
pembrolizumab results in the activation and proliferation of T lymphocytes with specificities
against tumor associated antigens (TILs). In untreated lung cancer tumors, we would expect
few tumors to have TIL cells with specificities against tumor associated antigens. Based on
the response rate to pembrolizumab in advanced lung cancer, we hypothesize that at least 20%
of lung cancers would have TIL cells with specificities against tumor associated antigens
after pembrolizumab therapy.

Studying neoadjuvant pembrolizumab therapy is an attractive strategy for studying the
immunologic changes caused by PD-1 (programmed death receptor 1) checkpoint masking. Most of
the immunologic activity associated with pembrolizumab occurs in the tumor and surrounding
microenvironment. Evaluation of post-pembrolizumab tumor will be important to understanding
factors associated with pembrolizumab activity, immune tolerance, and discovery of other
targets for immune therapy. Pembrolizumab has known benefit in non-small cell lung cancer.
The addition of pembrolizumab for two doses prior to surgery and four doses after surgery has
the potential to confer clinical benefit. Large randomized phase 3 trials are now testing
whether PD-1 checkpoint antibodies improve survival as adjuvant therapy after resection of
early stage lung cancer.

Inclusion Criteria:

- Histologically or cytologically confirmed NSCLC.

- Clinical stage IB (>/= 3 cm per CT), Stage IIA/IIB, or Stage IIIA (N0-2) amenable to
surgical resection.

- Primary tumor >/= 3 cm (for all stages entered) to make it likely that excess tumor
will be available after resection.

- Patient must be deemed a surgical candidate.

- ECOG performance status of 0 or 1 (Appendix C).

- No prior chemotherapy, radiation therapy or biologic/targeted therapy for current
diagnosis of lung cancer.

- Adequate Organ Function

- Age ≥18 years.

- Non-pregnant. Females of child-bearing potential (not surgically sterilized or
postmenopausal [a woman who is > 45 years of age and has not had menses for greater
than 1 year]) must test negative for pregnancy within 48 hours prior to any initial
study procedure based on a serum pregnancy test.

- No active invasive malignancy in the past 2 years other than non-melanoma skin cancer.
Cancers that are in-situ are not considered invasive.

- Signed written informed consent including HIPAA according to institutional guidelines.

- Patients must agree to research blood sampling to participate in study.

- Have measurable disease based on RECIST 1.1.

- Post-op predicted FEV1 and DLCO > 40% predicted (or per institutional standard).

Exclusion Criteria:

- Treatment within the last 30 days with a drug that has not received regulatory
approval for any indication at the time of study entry or used an investigational
device within 4 weeks of the first dose of treatment.

- Has a known history of active TB (Bacillus Tuberculosis).

- Hypersensitivity to pembrolizumab or any of its excipients.

- Concurrent administration of any other anti-tumor therapy.

- Has received prior therapy with an anti-PD-1, anti-PD-L-1, or anti-PD-L2 agent.

- Has known active Hepatitis B (e.g., HBsAg reactive) or Hepatitis C (e.g., HCV RNA
[qualitative] is detected).

- Inability to comply with protocol or study procedures.

- Active infection requiring antibiotics, antifungal or antiviral agents, that in the
opinion of the investigator would compromise the patient's ability to tolerate
therapy.

- Has known history of, or any evidence of active, non-infectious pneumonitis.

- Has a known history of Human Immunodeficiency Virus (HIV) (HIV 1/2 antibodies).

- Has active autoimmune disease that has required systemic treatment in the past 2 years
(i.e. with use of disease modifying agents, corticosteroids or immunosuppressive
drugs). Replacement therapy (e.g. thyroxine, insulin or physiologic corticosteroid
replacement therapy for adrenal or pituitary insufficiency etc) is not considered a
form of systemic treatment. Patients with a history of inflammatory bowel disease,
including ulcerative colitis and Crohn's Disease, are excluded from this study, as are
patients with a history of symptomatic disease (e.g., rheumatoid arthritis, systemic
progressive sclerosis [scleroderma], systemic lupus erythematosus, autoimmune
vasculitis [eg, Wegener's Granulomatosis]); motor neuropathy considered of autoimmune
origin (e.g. Guillain-Barre Syndrome and Myasthenia Gravis).

- Has a diagnosis of immunodeficiency or is receiving systemic steroid therapy or any
other form of immunosuppressive therapy within 7 days prior to the first dose of trial
treatment.

- Has a known additional invasive malignancy that is progressing or requires active
treatment. Exceptions include basal cell carcinoma of the skin or squamous cell
carcinoma of the skin that has undergone potentially curative therapy or in situ
cervical cancer.

- Is pregnant or breastfeeding, or expecting to conceive or father children within the
projected duration of the trial, starting with the pre-screening or screening visit
through 120 days after the last dose of trial treatment.

- Has had major surgery (other than definitive lung cancer surgery) within two weeks of
study or other serious concomitant disorders that in the opinion of the investigator
would compromise the safety of the patient or compromise the patient's ability to
complete the study.

- Has received any non-oncology vaccine therapy used for prevention of infectious
diseases (for up to 30 days before or after any dose of pembrolizumab). Note: Seasonal
influenza vaccines for injection are generally inactivated flu vaccines and are
allowed; however, intranasal influenza vaccines (e.g., Flu-Mist®) are live attenuated
vaccines and are not allowed.

- Has history of myocardial infarction having occurred less than 6 months before
inclusion, any known uncontrolled arrhythmia, symptomatic angina pectoris, active
ischemia, or cardiac failure not controlled by medications. Patients with CAD recently
treated with surgery and/or stent, if stable without symptomatic angina pectoris,
active ischemia are eligible.

- Has a history of interstitial lung disease

- Has known psychiatric or substance abuse disorders that would interfere with
cooperation with the requirements of the trial.

- Prisoners or subjects who are compulsorily detained involuntarily incarcerated) for
treatment of either psychiatric or physical (e.g., infectious) illness.