Differential Metabolism of Dietary Fatty Acids
| Status: | Completed |
|---|---|
| Conditions: | High Cholesterol, Obesity Weight Loss |
| Therapuetic Areas: | Cardiology / Vascular Diseases, Endocrinology |
| Healthy: | No |
| Age Range: | 18 - 35 |
| Updated: | 10/14/2017 |
| Start Date: | September 2000 |
| End Date: | April 2005 |
Differences in how diet fats are converted to energy could explain some of the reported
differences in health effects among different classes of dietary fat (e.g. monounsaturated
vs. saturated). Recently, this laboratory showed that monounsaturated fats are turned into
energy more readily than saturated fats. These results may mean that if one feeds more
monounsaturated fatty acids (MUFA) and less saturated fatty acids (SFA) in the diet, body fat
might accumulate at a lower rate. This could affect the risk of obesity and Type 2 Diabetes.
This project has two principal Specific Aims which will be assessed in healthy young adults
who are fed liquid formulas containing either an approximately equal amount of MUFA and SFA
(controls) or a much greater amount of MUFA and much less SFA:
1. To determine if a higher intake of MUFA and a reciprocally lower intake of SFA is
associated with a higher rate of fat oxidation. We hypothesize that the rate of fat
oxidation after eating will be higher in those subjects randomized to the MUFA-enriched
diet compared to controls.
2. To measure energy intake required to maintain constant body weight during each diet and
to measure fat-free mass and fat mass, before and after each dietary change. We
hypothesize that those on the high MUFA diet will need a higher energy intake required
to maintain constant body weight.
differences in health effects among different classes of dietary fat (e.g. monounsaturated
vs. saturated). Recently, this laboratory showed that monounsaturated fats are turned into
energy more readily than saturated fats. These results may mean that if one feeds more
monounsaturated fatty acids (MUFA) and less saturated fatty acids (SFA) in the diet, body fat
might accumulate at a lower rate. This could affect the risk of obesity and Type 2 Diabetes.
This project has two principal Specific Aims which will be assessed in healthy young adults
who are fed liquid formulas containing either an approximately equal amount of MUFA and SFA
(controls) or a much greater amount of MUFA and much less SFA:
1. To determine if a higher intake of MUFA and a reciprocally lower intake of SFA is
associated with a higher rate of fat oxidation. We hypothesize that the rate of fat
oxidation after eating will be higher in those subjects randomized to the MUFA-enriched
diet compared to controls.
2. To measure energy intake required to maintain constant body weight during each diet and
to measure fat-free mass and fat mass, before and after each dietary change. We
hypothesize that those on the high MUFA diet will need a higher energy intake required
to maintain constant body weight.
Our Preliminary Data indicate that the rate of oleic acid (OA) oxidation (amount/unit time)
is 21% increased compared to palmitic acid (PA) when both fatty acids are fed to the same
person at the same intake. We hypothesize that if the ratio of OA to PA in the diet were to
increase, the rate of total fatty acid oxidation in the fed state also would increase under
the same metabolic conditions. If our principal hypothesis is correct, overall daily fat
balance might be decreased in humans fed diets enriched in OA. This would have potential
significance to the treatment and prevention of obesity.
In order to address this hypothesis, we propose the following double-masked, randomized
control trial. Indirect calorimetry will be performed in the fed and fasting state in young,
healthy adults who will be studied under two conditions: after a 30-day, supervised, solid
food diet and then again after a 30-day formula diet. The subjects will be randomized to
receive either a Control Formula Diet of the same macronutrient and fatty acid composition as
the solid food diet (OA=PA=16.4% total kcal) or a High Oleic Acid Formula Diet (OA=31.4%
total kcal and PA=1.7% total kcal). The Control Diets (solid and liquid) provide amounts (per
kcal) of total fat, saturated fat, and OA that are similar to the to the 50-75th percentile
of intake of young adult Americans but above the intake of total fat and saturated fat that
is recommended for optimal long-term health. The High OA Formula Diet is designed to simulate
the fat source of the Mediterranean Diet. The High OA diet will result in a 167% increase in
the intake/kcal of OA in the sn-1 and sn-3 positions of the dietary triacylglycerol. Using
dual-energy x-ray absorptiometry (DEXA), we will monitor body composition before and after
each diet, while adjusting energy intake to maintain constant body weight. The following
Specific Aims will be addressed:
1. To determine if a higher intake of oleic acid (and a reciprocally lower intake of
palmitic acid) is associated with a higher rate of fat oxidation. We hypothesize that
the rate of fat oxidation (g/hr) in the fed state, adjusted for the covariate effect of
the rate of fat oxidation on the solid food diet, will be higher (>30%) in those
subjects randomized to the OA-enriched diet compared to controls. Furthermore, we
hypothesize that fat oxidation will not be higher when expressed as a proportion of
energy expenditure in the OA-enriched formula group. That is, we expect energy
expenditure in the fed state to be higher in those fed the OA-enriched diet.
2. To measure energy intake required to maintain constant body weight during each diet and
to measure fat-free mass and fat mass, before and after each dietary change. We
hypothesize that a higher rate of fat oxidation on the high OA diet will be associated
with a higher energy intake required to maintain constant body weight.
3. To compare fat oxidation on the liquid formula diet with that observed on the solid food
diet. We hypothesize that fat oxidation will increase in those fed the OA-enriched diet.
4. To measure the thermic effect of feeding during both the solid food and formula diet
periods. We hypothesize that the high OA feeding will be associated with a higher
thermic effect of feeding.
is 21% increased compared to palmitic acid (PA) when both fatty acids are fed to the same
person at the same intake. We hypothesize that if the ratio of OA to PA in the diet were to
increase, the rate of total fatty acid oxidation in the fed state also would increase under
the same metabolic conditions. If our principal hypothesis is correct, overall daily fat
balance might be decreased in humans fed diets enriched in OA. This would have potential
significance to the treatment and prevention of obesity.
In order to address this hypothesis, we propose the following double-masked, randomized
control trial. Indirect calorimetry will be performed in the fed and fasting state in young,
healthy adults who will be studied under two conditions: after a 30-day, supervised, solid
food diet and then again after a 30-day formula diet. The subjects will be randomized to
receive either a Control Formula Diet of the same macronutrient and fatty acid composition as
the solid food diet (OA=PA=16.4% total kcal) or a High Oleic Acid Formula Diet (OA=31.4%
total kcal and PA=1.7% total kcal). The Control Diets (solid and liquid) provide amounts (per
kcal) of total fat, saturated fat, and OA that are similar to the to the 50-75th percentile
of intake of young adult Americans but above the intake of total fat and saturated fat that
is recommended for optimal long-term health. The High OA Formula Diet is designed to simulate
the fat source of the Mediterranean Diet. The High OA diet will result in a 167% increase in
the intake/kcal of OA in the sn-1 and sn-3 positions of the dietary triacylglycerol. Using
dual-energy x-ray absorptiometry (DEXA), we will monitor body composition before and after
each diet, while adjusting energy intake to maintain constant body weight. The following
Specific Aims will be addressed:
1. To determine if a higher intake of oleic acid (and a reciprocally lower intake of
palmitic acid) is associated with a higher rate of fat oxidation. We hypothesize that
the rate of fat oxidation (g/hr) in the fed state, adjusted for the covariate effect of
the rate of fat oxidation on the solid food diet, will be higher (>30%) in those
subjects randomized to the OA-enriched diet compared to controls. Furthermore, we
hypothesize that fat oxidation will not be higher when expressed as a proportion of
energy expenditure in the OA-enriched formula group. That is, we expect energy
expenditure in the fed state to be higher in those fed the OA-enriched diet.
2. To measure energy intake required to maintain constant body weight during each diet and
to measure fat-free mass and fat mass, before and after each dietary change. We
hypothesize that a higher rate of fat oxidation on the high OA diet will be associated
with a higher energy intake required to maintain constant body weight.
3. To compare fat oxidation on the liquid formula diet with that observed on the solid food
diet. We hypothesize that fat oxidation will increase in those fed the OA-enriched diet.
4. To measure the thermic effect of feeding during both the solid food and formula diet
periods. We hypothesize that the high OA feeding will be associated with a higher
thermic effect of feeding.
- Healthy young adults, ages 18-35 yr. .: Generally, this means that the subject is not
being continually treated with medication (possible exceptions include thyroid
replacement therapy and some allergy treatments).
- Must avoid pregnancy during the study duration (we will test for this as part of the
protocol).
- Must avoid use of drugs that may affect lipid metabolism, including caffeine and
nicotine.
We found this trial at
1
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