Inhaled Nitric Oxide After Out-of-Hospital Cardiac Arrest

Subjects will be identified upon emergency department (ED) arrival or upon transfer from an
outside facility and screened for enrollment by a PCAS physician as soon as possible.
Eligible patients will have receive any required resuscitation (including central venous and
arterial line placement, endotracheal intubation and hemodynamic resuscitation as needed)
prior ot having baseline labs and studies performed. Subjects will then be started on study
drug delivered via the mechanical ventilator with a concealed canister (subject, providers
and outcome assessors blind to treatment assignment). Randomization will be performed 1:1 in
blocks of 8 using a random number generator. A randomization list will be prepared in advance
by the director of respiratory therapy (RT) at each site and verified by a company
representative. These individuals (who are not part of the study team) will be unblinded and
assure that allocation to placebo and intervention is accurate. The allocation list will
assign study drug canisters (by barcode) to each subject in order of study ID. Treatment
assignment will be revealed after opening a sealed opaque envelope once enrollment is
confirmed by a physician investigator. Study drug will then be administered by RT based on
allocation. During study drug administration hourly vital signs will be obtained and
methemoglobin levels for safety. Study drug will be weaned off after 12h over the course of
1h (10, 5, 4, 3, 2, 1 ppm each for 10 min) prior to discontinuation. Subjects will then
receive standard post-resuscitation care with outcomes assessed by a blinded study team
member (see below for outcomes).

Additional clinical variables to be collected Demographics and baseline function. Age, sex,
race, maximum education level, employment status, marital status, Barthel activities of daily
living (ADL) index prior to OHCA.

Arrest data. Location of OHCA, witnessed, bystander cardiopulmonary resuscitation (CPR),
estimated no flow and low flow times, presenting rhythm, doses of epinephrine administered,
shocks administered, recurrent arrest, date and time of ROSC.

Medical comorbidities. Diabetes, hypertension, active smoking, hyperlipidemia, chronic
obstructive pulmonary disease (COPD), hypertension, drug abuse, prior myocardial infarction,
prior coronary artery bypass grafting (CABG), prior coronary angiography with angioplasty or
stent, congestive heart failure (EF on last echocardiogram [ECHO] prior to OHCA), obstructive
sleep apnea, pulmonary hypertension, calculated Charlson comorbidity index (CCI).

Home medications. Statins, nitrates, anticoagulation, antiplatelet agents Hospital
Interventions. Coronary angiography, percutaneous coronary intervention, CABG, mechanical
ventilation hours and fraction of inspired oxygen (FiO2) from 0-24h after therapy start
In-hospital medications. Alteplase, anti-epileptic medication use (valproate, phenytoin,
lacosamide, leveteracetam), neurostimulants (methylphenidate, bromocriptine, modafinil,
amantadine), cumulative dose of fentanyl, propofol, midazolam, cis-atracurium and vecuronium
in at 24 (+/- 12 hours), 48 (+/- 12 hours), and 72 (+/- 12 hours) hours after therapy
initiation

Data Storage Subjects will be assigned a study identifier (ID) upon entry and all
data/samples stored using that ID. Linkage to patient identifiers will be maintained in a
secure spreadsheet and will include name, date of birth and medical record number. Clinical
data will be entered on case report forms (source documentation) which will be stored in a
locked filing cabinet within a locked office assigned to the study team. Deidentified
clinical and lab data will all be subsequently entered from the case report forms into a web
based database (REDCap) to be maintained by Dr. Dezfulian's research assistant who has prior
experience from other studies.

Statistical Analysis Plan Continuous data will be compared using t-tests and repeated
measures ANOVA to compare between iNO and placebo groups at multiple times. Dichotomous
outcomes including the primary endpoint will be compared by chi squared test. Time to
awakening and 90d survival will be compared by log rank test of Kaplan-Meier survival plots.
All tests will be two tailed with unadjusted p<0.05 considered significant. In the event of a
differential distribution of baseline variables strongly associated with outcome (univariate
OR >2), dichotomous outcomes will be adjusted for these baseline variables.

Inclusion Criteria:

- Intubated and comatose adult (>18 yo) resuscitated from out-of-hospital cardiac arrest
(OHCA)*

*Cardiac arrest within an emergency department or outpatient medical center will be
included). OHCA includes Emergency Medical Service (EMS) witnessed cardiac arrest.

- Return of spontaneous circulation (ROSC) within 40 min of CPR initiation

- Full Outline of Unresponsiveness (FOUR) Brainstem score ≥ 2 (i.e. patient must have
pupil OR corneal reflex at the time of ED presentation or within 1h if
sedation/neuromuscular blockade clouds the picture)

Exclusion Criteria:

- Traumatic etiology of OHCA

- Prisoner

- Known pregnancy (beta-human chorionic gonadotropin screening is NOT REQUIRED for
enrollment in women of appropriate age)

- Hemodynamic instability defined as >1 recurrent arrest prior to enrollment OR
inability to maintain mean arterial blood pressure (MAP) > 65 using vasopressors and
inotropes (ie actively up titrating medications or giving fluid bolus)

- Head CT grey-white ratio < 1.2; Head CT is NOT REQUIRED prior to enrollment

- Fixed and dilated pupils without another explanation

- Known intracranial hemorrhage or acute cerebral infarction; Head CT is NOT REQUIRED
prior to enrollment

- Malignant EEG upon presentation defined as: myoclonic status epilepticus,
non-convulsive status epilepticus, generalized periodic epileptiform discharges. EEG
screening is NOT REQUIRED prior to enrollment

- ROSC >3h from time of ED arrival (treatment allocation must be within 4h so anything
that will prevent this is reason for exclusion)

- Alert and interactive patient with minimal evidence of neurologic injury

- Plan to extubate within 12 hours

- Post-cardiac arrest service (PCAS) physician opinion that patient will die with >95%
likelihood. This may be based on:

- Multiple medical comorbidities

- Late discovery of don not resuscitate (DNR) or advanced directive

- Terminal diagnosis (other than OHCA; may have caused OHCA)

- Clinical judgement based on current exam and data

- Patient is known to be taking phosphodiesterase type 5 (PDE5) inhibitors, soluble
guanylyl cyclase (sGC) stimulator, or has a known diagnosis of Chronic thromboembolic
pulmonary hypertension (CTEPH), pulmonary hypertension (PAH), or erectile dysfunction

- Known enrollment in another acute interventional study.