Efficacy and Safety of Fenugreek Extract on Markers of Muscle Damage and Inflammation in Untrained Males

Subjects expressing interest in doing the study will be interviewed in the UMHB Human
Performance Lab (HPL) to determine whether they qualify to participate in the study. Upon
qualification, participants will be scheduled a familiarization session in which they will be
taught how to perform a proper bench press, back squat, deadlift and squat thruster. They
will then schedule their first testing session which will include: a body composition
analysis via an InBody 770, thigh circumference measurements, joint mobility measurements, a
blood draw, strength testing (1RM), and muscular endurance testing (70% 1RM max reps).
Participants will then be placed in groups based on fat free mass and assigned their workout
program (3days/week) with training loads based on their 1RM values (bench press, back squat,
deadlift and squat thruster).

Supplementation protocol: Participants will be randomly assigned into one of three groups and
instructed to ingest capsules of either a placebo, or IND03 (fenugreek extract) containing
300mg, or 500mg. They will supplement 1 hour prior to their workout each exercise day as well
as during the overreaching week. On "off/rest" days, participants will consume capsules in
the morning with breakfast.

After two weeks of training, participants will return to the HPL for follow-up strength
testing (1RM), and muscular endurance testing (70% 1RM max reps), then instructed to rest for
48 hours and return to the lab to begin their overreaching protocol. During each of the 5
days, participants undergo the following measurements prior to the exercise protocol: thigh
circumference, joint mobility, and questionnaires (WOMAC & VAS). On Day 1 participants will
also perform an InBody and blood draw. A blood draw will also be performed on Day 3. They
will then consume a standardized snack and begin the overreaching protocol of 8 sets of 10
repetitions for the following 4 exercises: bench press, back squat, deadlift and squat
thruster. There will be a 1 minute rest period in between each set and a 2 minute rest in
between each exercise. Participants will then fill out a Rate of Perceived Exertion (RPE)
immediately after the protocol. Again, they will perform this for 5 consecutive days.

Twenty-four hours after the last overreaching day, participants will return for thigh
circumference, joint mobility, questionnaires (WOMAC & VAS), a blood draw, strength testing
(1RM), and muscular endurance testing (70% 1RM max reps). They will then be instructed to
continue the resistance training program for 1 week. After that period they will return for
final measurements of thigh circumference, joint mobility, questionnaires (WOMAC & VAS), a
blood draw, strength testing (1RM), and muscular endurance testing (70% 1RM max reps).

Throughout the duration of the study, participants will record their dietary intake via
MyFitnessPal.

Inclusion Criteria:

- Subjects will be males between the age of 18-45;

- Subjects will have not been participating in a structured resistance training program
in the past 6 months;

- Subjects will be provided written and dated informed consent to participate in the
study;

- Subjects will be willing and able to comply with the protocol;

- Subjects will be apparently healthy and free from disease, as determined by a health
history questionnaire;

- Subjects will agree to abstain from exercise 48 hours prior to each testing visit;

- Subjects will agree to fast for 10 hours prior to each testing visit;

- Subjects will be agree to refrain from tobacco use, alcohol and/or caffeine
consumption and/or smoking 12 hours prior to each testing visit;

- Subjects will agree to refrain from taking any supplement that may interfere with
study supplementation for the remainder of the study.

Exclusion Criteria:

- Subject has taken ergogenic levels of nutritional supplements that may affect muscle
mass (e.g., creatine, HMB) or anabolic/catabolic hormone levels (e.g.,
androstenedione, DHEA, etc.) within 3 months prior to the start of the study;

- Subject is unable to complete blood draws needed at each testing session;

- Subject reports any unusual adverse events associated with this study that in
consultation with the supervising physician recommends removal from the study;

- Subject does not complete 90% of resistance training program assigned;

- Subject is unable to complete overreaching protocol during overreaching week;