Bilateral Orthotopic Lung Transplant - Bone Marrow Transplant

The primary purpose of this study is to evaluate the safety and efficacy of performing
bilateral orthotopic lung transplantation (BOLT) followed by cadaveric, partially HLA-matched
CD3+/CD19+-depleted hematopoietic stem cell transplantation (HSCT) from the same donor for
participants with primary immunodeficiency diseases (PID) and end-stage lung disease. For
many patients with primary immunodeficiencies, HSCT, which we refer to as Bone Marrow
Transplant (BMT) is a curative, life-saving therapy, resulting in restoration of function in
the immune system. Patients with primary immunodeficiencies often develop pulmonary
complications as a result of chronic or recurrent infections, making them ineligible for BMT
due to the high risk of mortality and pulmonary complications. Lung transplant prior to BMT
would allow for restoration of pulmonary function prior to BMT, allowing PID patients to
proceed to BMT , which would be curative for the patient's underlying immunodeficiency. As a
secondary aim, after successful engraftment with donor bone marrow, the feasibility of
participants tolerating planned withdrawal of immunosuppression and achieving eventual
freedom from all immunosuppressive drugs and attaining a tolerant state will be assessed.

This is a single center study in which participants receive a cadaveric, partially Human
Leukocyte Antigen (HLA)-matched lung transplant followed by a CD3+/CD19+ depleted bone marrow
transplant (BMT) from the same donor. In this study, the investigators will use a ≥ 2/6
HLA-matched T cell depleted bone marrow transplant from a cadaveric organ donor with an
identical ABO blood type as the recipient.

Participants will undergo:

- Bilateral orthotopic lung transplant (BOLT) utilizing basiliximab induction or an
alternate induction therapy based on their underlying disease. Rituximab may be
initiated prior to the lung transplant, with tacrolimus as the ongoing maintenance
immunosuppression.

- BMT utilizing CD3+/CD19+-depleted bone marrow with bone marrow conditioning beginning no
less than 8 weeks after BOLT.

The duration of participant involvement in the trial is up to 2 years post-BMT.

Inclusion Criteria:

- Subject and/or parent guardian must be able to understand and provide informed
consent;

- Subject fulfills criteria for United Network of Organ Sharing (UNOS) listing;

- Subject must have evidence of an underlying primary immunodeficiency for which Bone
Marrow Transplant (BMT) is clinically indicated. Examples of such diseases include,
but are not limited to:

- Severe Combined Immunodeficiency (SCID)

- Combined immunodeficiency with defects in T-cell-mediated immunity, including
Omenn syndrome and DiGeorge Syndrome

- Severe Chronic Neutropenia

- Chronic Granulomatous Disease (CGD)

- Hyper Immunoglobulin E (IgE) Syndrome or Job Syndrome

- CD40 or CD40L deficiency

- Wiskott-Aldrich Syndrome

- Mendelian Susceptibility to Mycobacterial Disease

- GATA2-associated Immunodeficiency.

- Subjects must have evidence of end-stage lung disease and be candidates for bilateral
orthotopic lung transplant as determined by the lung transplant team;

- Glomerular filtration rate (GFR) ≥ 50 mL/min/1.73 m^2;

- Aspartate aminotransferase (AST), Alanine aminotransaminase (ALT) ≤ 4x upper limit of
normal, total bilirubin ≤ 2.5 mg/dL, normal INR;

- Cardiac ejection fraction ≥ 40% or shortening fraction ≥ 26%;

- Negative pregnancy test for females >10 years old or who have reached menarche, unless
surgically sterilized;

- All females of childbearing potential and sexually active males must agree to use a
Food and Drug Administration (FDA) approved method of birth control for up to 24
months after BMT or for as long as they are taking any medication that may harm a
pregnancy, an unborn child or may cause birth defect; and

- Subject and/or parent guardian will also be counseled regarding the potential risks of
infertility following BMT and advised to discuss sperm banking or oocyte

- harvesting.

Eligibility for Bone Marrow Transplant*:

- GFR >50 mL/min/1.73 m^2;

- AST, ALT <4x upper limit of normal, Total bilirubin < 2.5 mg/dL;

- Cardiac ejection fraction ≥40% or shortening fraction of at least 26%;

- Human Immunodeficiency Virus (HIV) negative by serology and PCR;

- Human T-lymphotropic virus (HTLV) serology negative;

- Forced vital capacity (FVC) and Forced expiratory volume (FEV1) ≥ 40% predicted for
age and SpO2 of >90% at rest on room air AND with clearance by the lung transplant
team;

- Absence of uncontrolled infection as determined by blood cultures and radiographic
results of previously affected sites, in particular, pulmonary densities during the
past 2 weeks prior to chemotherapy;

- Absence of Acute Cellular Rejection (ACR); and

- Bone marrow processing has been completed, and an appropriate stem cell product is
available for administration.

- Note: The decision to proceed with the BMT will be at the discretion of the lung
transplant team following clearance by the bone marrow team based on the criteria
below. The conditioning for the BMT will begin no less than 8 weeks following the
lung transplant.

Exclusion Criteria:

- Inability or unwillingness of a participant to give written informed consent or comply
with study protocol;

- Subjects who have underlying malignant conditions;

- Subjects who have non-malignant conditions that do not require hematopoietic stem cell
transplantation;

- Human Immunodeficiency Virus (HIV) positive by serology or polymerase chain reaction
(PCR), human T-lymphotropic virus (HTLV) positive by serology;

- Females who are pregnant or who are lactating;

- Allergy to dimethyl sulfoxide (DMSO) or any other ingredient used in the manufacturing
of the stem cell product;

- Uncontrolled pulmonary infection, as determined by radiographic findings and/or
significant clinical deterioration.

-- Pulmonary colonization with multiple organisms is common, and will not be
considered an exclusion criterion.

- Uncontrolled systemic infection, as determined by the appropriate confirmatory testing
e.g. blood cultures, PCR testing, etc.;

- Recent recipient of any licensed or investigational live attenuated vaccine(s), within
4 weeks of transplant; or

- Past or current medical problems or findings from physical examination or laboratory
testing that are not listed above, which, in the opinion of the investigator, may
pose:

- additional risks from participation in the study,

- may interfere with the participant's ability to comply with study requirements,

- or that may impact the quality or interpretation of the data obtained from the
study.