Trial to Evaluate the Effect of ALN-PCSSC Treatment on Low Density Lipoprotein Cholesterol (LDL-C)

Participants will be screened and 480 eligible participants will be randomized: 60
participants per each of six ALN-PCSSC dose groups plus 120 participants total across the
placebo groups (20 participants each to match each of the six drug dose groups). Treatment
allocation will be stratified by country and by current use of statins or other
lipid-modifying therapies. Each participant will receive either one or two injections on Day
1 or a single injection on Day 1 and on Day 90 of blinded ALN-PCSSC or placebo.

Formation of anti-drug antibodies (ADA) will be assessed on Day 1 (prior to and 4 hours after
the injection) and on Days 30, 60, 90, 120, 150, 180 (Days 150 and 180 only in participants
who receive a second dose of study drug), and 210 or until any ADA response becomes negative
within the study duration.

The independent Data Monitoring Committee (DMC) will review safety data beginning after the
first 40 participants receive the first injection of ALN-PCSSC or placebo and complete the
Day 14 follow-up visit. Thereafter, the DMC will review safety data every 2 months until the
end of the trial. A recommendation may be taken to stop or amend the study at any of these
reviews.

On Day 1, all eligible participants will be randomized and receive the first subcutaneous
(SC) administration of ALN-PCSSC or placebo. After the first study drug administration, the
participant will be observed in the clinic for at least 4 hours post injection before being
discharged. Participants will return at Day 14 and then at monthly intervals for 6 months.
Participants randomized to receive a second dose of study drug will receive the second
injection of ALN-PCSSC or placebo at the Day 90 visit.

Efficacy assessments will include the measurement of the effects of ALN-PCSSC on levels of
LDL-C lipids and lipoproteins including total cholesterol (TC), triglycerides, high-density
lipoprotein cholesterol (HDL-C), non-HDL-C, very low-density lipoprotein (VLDL),
apolipoprotein A1 (Apo-AI), apolipoprotein B (Apo-B), lipoprotein (a) [Lp(a)], C-reactive
protein (CRP), and proprotein convertase subtilisin/kexin type 9 (PCSK9).

End of study (EOS) evaluations will be conducted at the EOS visit (Day 210). The expected
duration of the participants' involvement in the study will be approximately 374 days, which
includes screening, study drug administration, the course of single or multiple injections,
and the follow-up period to Day 360.

Participants completing the study to Day 210 will be given the opportunity to enroll in a
separate long-term extension study. Any participants in whom LDL-C levels have not returned
to >80% of baseline values will continue to be followed as part of this study until either
this level has been reached or until a maximum of Day 360, at which point they will be given
the opportunity to enroll in the long-term extension study. At each visit, LDL-C levels,
adverse events, serious adverse events, concomitant medications, and safety laboratory
assessments will be collected.

Objectives:

Primary:

To evaluate the effect of ALN-PCSSC treatment on LDL-C levels at Day 180.

Secondary:

To evaluate the effect of ALN-PCSSC on the following:

- LDL-C at Day 90

- LDL-C levels at other time points

- PCSK9 levels over time

- Other lipids, lipoproteins, apolipoproteins

- Proportion of participants achieving pre-specified global lipid guidelines

- Individual responsiveness to different doses

- Duration of lipid-lowering effect of different doses

- Safety and tolerability profile of ALN-PCSSC

Exploratory:

To collect/evaluate the effect of ALN-PCSSC on the following:

- Cardiovascular (CV) events such as CV death, non-fatal myocardial infarction,
resuscitated cardiac arrest and non-fatal stroke (ischemic and hemorrhagic)

- Evaluation of ADA for the investigational product

Inclusion Criteria:

1. Male or female participants ≥18 years of age.

2. History of ASCVD or ASCVD-risk equivalents (symptomatic atherosclerosis, Type 2
diabetes, familial hypercholesterolemia, including participants whose 10-year risk of
a CV event assessed by Framingham Risk Score (Framingham Risk Score >20%) or
equivalent has a target LDL-C of <100 mg/deciliter [dL]).

3. Serum LDL-C ≥1.8 millimole (mmol)/liter (L) (≥70 mg/dL) for ASCVD participants or ≥2.6
mmol/L (≥100 mg/dL) for ASCVD-risk equivalent participants at screening.

4. Fasting triglyceride <4.52 mmol/L (<400 mg/dL) at screening.

5. Calculated glomerular filtration rate 30 mL/min or higher by estimated glomerular
filtration rate (eGFR) using standardized local clinical methodology.

6. Participants on statins should be receiving a maximally tolerated dose (investigator's
discretion).

7. Participants on lipid-lower therapies (such as statin and/or ezetimibe) should be on a
stable dose for ≥30 days before screening with no planned medication or dose change
during study participation.

8. Willing and able to give informed consent before initiation of any study-related
procedures and willing to comply with all required study procedures.

Exclusion Criteria:

1. Any uncontrolled or serious disease, or any medical or surgical condition, that may
either interfere with participation in the clinical study, and/or put the participant
at significant risk (according to investigator's [or delegate] judgment) if he/she
participates in the clinical study.

2. An underlying known disease, or surgical, physical, or medical condition that, in the
opinion of the investigator (or delegate), might interfere with interpretation of the
clinical study results.

3. New York Heart Association (NYHA) class II, III, or IV heart failure or last known
left ventricular ejection fraction <30%.

4. Cardiac arrhythmia within 3 months prior to randomization that is not controlled by
medication or via ablation.

5. Any history of hemorrhagic stroke.

6. Major adverse cardiac event within 6 months prior to randomization.

7. Uncontrolled severe hypertension: systolic blood pressure >180 millimeters of mercury
(mmHg) or diastolic blood pressure >110 mmHg prior to randomization despite
anti-hypertensive therapy.

8. Poorly controlled Type 2 diabetes, such as, glycated hemoglobin A1c (HbA1c)>10.0%
prior to randomization.

9. Active liver disease defined as any known current infectious, neoplastic, or metabolic
pathology of the liver or unexplained alanine aminotransferase (ALT) or aspartate
aminotransferase (AST) elevation >2x the upper limit of normal (ULN), or total
bilirubin elevation >1.5x ULN at screening confirmed by a repeat measurement at least
1 week apart.

10. Serious comorbid disease in which the life expectancy of the participant is shorter
than the duration of the trial (for example, acute systemic infection, cancer, or
other serious illnesses). This includes all cancers with the exception of treated
basal-cell carcinoma occurring >5 years before screening.

11. Females who are pregnant or nursing, or who are of childbearing potential and
unwilling to use at least two methods of contraception (oral contraceptives, barrier
methods, approved contraceptive implant, long-term injectable contraception,
intrauterine device or tubal litigation) for the entire duration of the study. Women
who are >2 years postmenopausal defined as ≥1 year since last menstrual period and if
less than 55 years old with a negative pregnancy test within 24 hours of randomization
or surgically sterile are exempt from this exclusion.

12. Males who are unwilling to use an acceptable method of birth control during the entire
study period (such as, condom with spermicide).

13. Known history of alcohol and/or drug abuse within the last 5 years.

14. Treatment with other investigational medicinal products or devices within 30 days or
five half˗lives, whichever is longer.

15. Use of other investigational medicinal products or devices during the course of the
study.

16. Any condition that according to the investigator could interfere with the conduct of
the study, such as but not limited to the following:

- Inappropriate for this study, including participants who are unable to
communicate or to cooperate with the investigator.

- Unable to understand the protocol requirements, instructions and study-related
restrictions, the nature, scope, and possible consequences of the study
(including participants whose cooperation is doubtful due to drug abuse or
alcohol dependency).

- Unlikely to comply with the protocol requirements, instructions, and
study-related restrictions (for example, uncooperative attitude, inability to
return for follow-up visits, and improbability of completing the study).

- Have any medical or surgical condition, which in the opinion of the investigator
would put the participants at increased risk from participating in the study.

- Involved with, or a relative of, someone directly involved in the conduct of the
study.

- Any known cognitive impairment (for example, Alzheimer's disease)

17. Previous or current treatment (within 90 days of screening) with monoclonal antibodies
directed at PCSK9.