The Effect of Sitting and Moderate Exercise on Plasma Triglyceride Elevation After a Meal

Subjects will undergo three weeks (two intervention, one washout) of testing during which
they will participate in two conditions: 1) prolonged sitting (~14-hours/day) with a 1-hour
bout of acute exercise at 65% of pre-intervention VO2max and 2) prolonged sitting
(~14-hours/day) without an acute bout of exercise. During each intervention week, subjects
will have 2 days of dietary and activity control, followed by 4 days during which prolonged
sitting will occur. On the evening of the 4th day, subjects in the exercise group will
perform the acute bout of exercise. On day 5, all subjects will undergo a lipid tolerance
test to determine the body's ability to clear triglycerides from the blood as well as
insulin/glucose response and substrate oxidation. Blood samples will be assayed for the
previously mentioned substances and postprandial gas collection will be analyzed via indirect
calorimetry.

Inclusion Criteria:

Exclusion Criteria:

- cardiovascular problems (e.g. pre-existing heart issues, coronary artery disease,
hypertension, etc.)

- respiratory problems

- musculoskeletal problems that prevent prolonged sitting or exercise

- obesity

- susceptibility to fainting