Effect of Orally Delivered Phytochemicals on Aging and Inflammation in the Skin
| Status: | Recruiting |
|---|---|
| Healthy: | No |
| Age Range: | 18 - 70 |
| Updated: | 12/22/2018 |
| Start Date: | September 25, 2017 |
| End Date: | December 1, 2019 |
| Contact: | Jed W Fahey, ScD |
| Email: | jfahey@jhmi.edu |
| Phone: | 410-614-2607 |
Effect of Orally Delivered Phytochemicals, Alone, and in Combination, on Aging and Inflammation-Related Effects in the Skin
The objective is to determine, in a small number of participants, the protective effects of
UV-induced skin erythema (reddening or "sunburn") following oral administration of
sulforaphane, curcumin, or a combination of the two plant (diet)-based supplements. The
investigators will be using the over-the-counter nutritional supplements Crucera-SGS and
Meriva-SF to deliver the biologically stable sulforaphane precursor and a highly bioavailable
formulation of curcumin. Volunteers will be challenged with UV irradiation at 2-times the
minimum erythematous dose (M.E.D.) on discrete 2 cm diameter circles on the upper buttocks.
Skin redness will be monitored daily using a chromometer. Biomarkers will then be evaluated
in blood, urine, and skin biopsies.
UV-induced skin erythema (reddening or "sunburn") following oral administration of
sulforaphane, curcumin, or a combination of the two plant (diet)-based supplements. The
investigators will be using the over-the-counter nutritional supplements Crucera-SGS and
Meriva-SF to deliver the biologically stable sulforaphane precursor and a highly bioavailable
formulation of curcumin. Volunteers will be challenged with UV irradiation at 2-times the
minimum erythematous dose (M.E.D.) on discrete 2 cm diameter circles on the upper buttocks.
Skin redness will be monitored daily using a chromometer. Biomarkers will then be evaluated
in blood, urine, and skin biopsies.
Oral sulforaphane (SF) delivery results in systemic protection of a wide variety of organ
systems which The investigators hypothesize will also include the skin, based on animal
studies and preliminary evidence in human volunteers. Since The investigators have only
previously evaluated the ability of topical sulforaphane application to protect skin from
UV-induced erythema, the next logical step is to evaluate the ability of oral delivery to
affect the skin of healthy human volunteers. Curcumin is also a potent anti-inflammatory that
acts upon different biochemical pathways from SF, and it is an antioxidant. It was discovered
over a century ago, it has been the subject of well over a hundred clinical studies, and it
has been an ingredient in common foods eaten by millions of people, for centuries. Before and
after consumption of each of these common food ingredients, The investigators will: (a)
measure the Phase 2 cytoprotective response in human skin, (b) determine whether it leads to
reduced UV-induced erythema (reduced inflammation), (c) evaluate changes in age-related
markers such as dermal elasticity, keratin and collagen levels, (d) measure advanced
glycation end-products (AGEs) in the serum, as biomarkers of systemic (including the skin)
reduction in AGE levels, and (e) measure the levels of these biomarkers in skin punch
biopsies. The investigators will also evaluate the effects of combined oral SF and curcumin.
The investigators anticipate that there may be a true synergistic response between SF and
curcumin, and the experiments designed herein are designed to show that synergy, if it
exists.
systems which The investigators hypothesize will also include the skin, based on animal
studies and preliminary evidence in human volunteers. Since The investigators have only
previously evaluated the ability of topical sulforaphane application to protect skin from
UV-induced erythema, the next logical step is to evaluate the ability of oral delivery to
affect the skin of healthy human volunteers. Curcumin is also a potent anti-inflammatory that
acts upon different biochemical pathways from SF, and it is an antioxidant. It was discovered
over a century ago, it has been the subject of well over a hundred clinical studies, and it
has been an ingredient in common foods eaten by millions of people, for centuries. Before and
after consumption of each of these common food ingredients, The investigators will: (a)
measure the Phase 2 cytoprotective response in human skin, (b) determine whether it leads to
reduced UV-induced erythema (reduced inflammation), (c) evaluate changes in age-related
markers such as dermal elasticity, keratin and collagen levels, (d) measure advanced
glycation end-products (AGEs) in the serum, as biomarkers of systemic (including the skin)
reduction in AGE levels, and (e) measure the levels of these biomarkers in skin punch
biopsies. The investigators will also evaluate the effects of combined oral SF and curcumin.
The investigators anticipate that there may be a true synergistic response between SF and
curcumin, and the experiments designed herein are designed to show that synergy, if it
exists.
Inclusion Criteria:
- 18-70 years old, healthy
- Willingness to avoid sun exposure to study site
- Willingness to adhere to cruciferous vegetable-free diet
Exclusion Criteria:
- Use of photosensitizing medications
- Use of medications that cause skin flushing
- Use of anticoagulants/antiplatelet therapies
- Allergies to anesthetic agents
- Use of systemic retinoids or steroids (excluding female contraceptives and
levothyroxin)
- Topical retinoids or steroids at study sites
- Antibiotic use
- Current students of the Principal Investigator
- Procedures performed at the study sites
- Smokers/tobacco users
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