The Molecular Basis for a Broad Range of Phenotypes Related to hTAS2R38
| Status: | Completed |
|---|---|
| Conditions: | Healthy Studies |
| Therapuetic Areas: | Other |
| Healthy: | No |
| Age Range: | 18 - 40 |
| Updated: | 12/20/2017 |
| Start Date: | July 2011 |
| End Date: | June 2013 |
This single-site, within-subject, experimental study is designed to test the hypothesis that
individuals express taste receptors differently and have different sensitivities to bitter
taste samples, which results from uneven expression. Subjects will include 100 racially and
ethnically diverse, healthy adults. All subjects will provide saliva samples for genetic
analysis and undergo taste testing with common psychophysical stimuli. From the pool of 100
subjects, 36 subjects from the common heterozygous group (PAV/AVI) and two from each
homozygous group (AVI/AVI, nontaster and PAV/PAV, taster) will be invited to return for two
additional sessions. During both sessions the investigators will 1) measure bitterness
thresholds for PROP, a common psychophysical stimulus; 2) measure bitterness perception of
several vegetables; 3) obtain a saliva sample and 4) collect taste papillae. The
investigators will then isolate DNA and RNA from the taste papillae and isolate DNA from
saliva to evaluate molecular differences over time and between individuals and how it relates
to taste psychophysics. All psychophysical and cellular measurements will be done during both
sessions so that the investigators can determine how much variation is observed over a
one-week time period in both cellular phenotype and psychophysical phenotype.
individuals express taste receptors differently and have different sensitivities to bitter
taste samples, which results from uneven expression. Subjects will include 100 racially and
ethnically diverse, healthy adults. All subjects will provide saliva samples for genetic
analysis and undergo taste testing with common psychophysical stimuli. From the pool of 100
subjects, 36 subjects from the common heterozygous group (PAV/AVI) and two from each
homozygous group (AVI/AVI, nontaster and PAV/PAV, taster) will be invited to return for two
additional sessions. During both sessions the investigators will 1) measure bitterness
thresholds for PROP, a common psychophysical stimulus; 2) measure bitterness perception of
several vegetables; 3) obtain a saliva sample and 4) collect taste papillae. The
investigators will then isolate DNA and RNA from the taste papillae and isolate DNA from
saliva to evaluate molecular differences over time and between individuals and how it relates
to taste psychophysics. All psychophysical and cellular measurements will be done during both
sessions so that the investigators can determine how much variation is observed over a
one-week time period in both cellular phenotype and psychophysical phenotype.
Inclusion Criteria:
- Key inclusion criteria include age 18-40 years; English speaking, and good health at
the time of study participation.
Exclusion Criteria:
- Key exclusion criteria include cigarette smoking, which alters taste perception, oral
disease and subjects undergoing Coumadin therapy. Subjects showing signs of oral
disease, including tongue lesions or xerostomia will be excluded from tongue biopsy
and therefore excluded from the study. Subjects undergoing Coumadin therapy will be
excluded from tongue biopsy due to the risk of bleeding, and therefore excluded from
the study. Subjects will not be excluded because of economic status, gender, race or
ethnicity.
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