A New Optical Sweat Test Method Based on a Citric Acid-derived Multi-halide Sensor

Cystic Fibrosis (CF) is a hereditary multi-system disease affecting approximately 30,000n
children and adults in the USA. The diagnosis of CF requires biochemical confirmation (either
abnormal sweat chloride measurement and/or identification of two CF disease causing
mutations) plus clinical symptomatology. Measurements of sweat chloride remain cumbersome and
although most common methodology to confirm CF diagnosis with limitations especially in young
children less than 6 months of age and in areas that lack ability for the complex testing.
The study objectives of this current research proposal include: A) To expand upon previously
obtained pilot study data "Evaluation of a fluorescent-based chloride sensor as an optical
sweat test to diagnose cystic fibrosis" B) To add the exploratory measurement of sweat
Bromide as a first in human assessment observation, C) To Evaluate the development of
smartphone based point-of-care technology for chloride and bromide sensor measurements, D) To
further expand the class of citrate-based sensors with improved fluorescence and sensing
properties for the design of new fluorescence-based analytical and diagnostic solutions based
on the automated multi-halide detection system, and E) To develop point-of-care systems that
can successfully integrate into clinical settings to improve current practices and facilitate
early detection of disease.