Evaluating the Infectivity, Safety, and Immunogenicity of a Respiratory Syncytial Virus Vaccine (RSV 6120/∆NS2/1030s) in RSV-Seropositive Children and RSV-Seronegative Infants and Children

This study will evaluate the infectivity, safety, and immunogenicity of a single dose of a
recombinant, live-attenuated RSV vaccine (RSV 6120/∆NS2/1030s) in RSV-seropositive children
12 to 59 months of age and RSV-seronegative infants and children 6 to 24 months of age.

The vaccine will be evaluated in a stepwise fashion beginning in RSV-seropositive children
(Group 1), and then in RSV-seronegative infants and children (Group 2). In each group,
participants will be randomly assigned to receive a single dose of RSV 6120/∆NS2/1030s
vaccine or placebo at study entry (Day 0).

Participants will be enrolled in the study between April 1 and October 31, outside of the RSV
season. Group 1 (RSV-seropositive children) will be followed for 28 days. Group 2
(RSV-seronegative infants and children) will remain on study until they complete the post-RSV
season visit between April 1 and April 30 in the calendar year following enrollment.

The expected initial study duration will be 28 days for Group 1 participants and between 6
and 13 months for Group 2 participants, depending on when they enroll in the study. Group 2
participants may participate in an optional second season of RSV surveillance during November
to March of the second year following enrollment. The expected study duration for these Group
2 participants will be up to 25 months.

Study visits for all participants may include clinical assessments, blood collection, and
nasal washes. Additionally, participants' parents or guardians will be contacted by study
staff at various times during the study to monitor participants' health.

Inclusion Criteria for RSV-Seropositive Children:

- Greater than or equal to 12 months of age and less than 60 months of age at the time
of inoculation

- Screening serum specimen for RSV neutralizing antibody is obtained within the calendar
year of inoculation

- Seropositive for RSV antibody, defined as serum RSV neutralizing antibody titer
greater than or equal to 1:40

- Pre-inoculation serum sample for RSV neutralizing antibody testing is obtained no more
than 56 days prior to inoculation

- In good health based on review of the medical record, history, and physical
examination at the time of inoculation

- Received routine immunizations appropriate for age based on the Advisory Committee on
Immunization Practices (ACIP) Recommended Immunization Schedule for Children and
Adolescents Aged 18 Years or Younger

- Growing normally for age as demonstrated on a standard growth chart and has a current
height and weight above the 3rd percentile for age

- Expected to be available for the duration of the study

- Parent/guardian is willing and able to provide written informed consent

Exclusion Criteria for RSV-Seropositive Children:

- Born at less than 34 weeks gestation

- Maternal history of positive HIV test

- Evidence of chronic disease

- Known or suspected impairment of immune function

- Bone marrow/solid organ transplant recipient

- Major congenital malformations, including congenital cleft palate or cytogenetic
abnormalities

- Suspected or documented developmental disorder, delay, or other developmental problem

- Cardiac abnormality requiring treatment

- Lung disease or reactive airway disease

- More than one episode of wheezing in the first year of life

- Wheezing episode or received bronchodilator therapy within the past 12 months

- Wheezing episode or received bronchodilator therapy after the age of 12 months

- Previous receipt of supplemental oxygen therapy in a home setting

- Previous receipt of an investigational RSV vaccine

- Previous receipt or planned administration of anti-RSV drug (ribavirin) or RSV
antibody product including RSV immune globulin (RSV Ig) or RSV monoclonal antibody
(RSV mAb)

- Previous receipt of immunoglobulin or any antibody products within the past 6 months

- Previous receipt of any other blood products within the past 6 months

- Previous anaphylactic reaction

- Previous vaccine-associated adverse reaction that was Grade 3 or above

- Known hypersensitivity to any vaccine component

- Member of a household that contains an infant who is less than 12 months of age at the
date of inoculation through the 10th day after inoculation

- Member of a household that, at the date of inoculation through the 10th day after
inoculation, contains an immunocompromised individual including but not limited to:

- a person who is HIV-infected

- a person who has received chemotherapy within the 12 months prior to enrollment

- a person receiving immunosuppressant agents

- a person living with a solid organ or bone marrow transplant

- Will attend a daycare facility that does not separate children by age and contains an
infant who is less than 12 months of age at the date of inoculation through the 10th
day after inoculation

- Receipt of any of the following prior to enrollment:

- any inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days
prior, or

- any live vaccine, other than rotavirus vaccine, within the 28 days prior, or

- another investigational vaccine or investigational drug within 28 days prior, or

- salicylate (aspirin) or salicylate-containing products within the past 28 days

- Scheduled administration of any of the following after planned inoculation:

- inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days
after, or

- any live vaccine other than rotavirus in the 28 days after, or

- another investigational vaccine or investigational drug in the 28 days after

- Receipt of any of the following medications within 3 days of study enrollment:

- systemic antibacterial, antiviral, antifungal, anti-parasitic, or antituberculous
agents, whether for treatment or prophylaxis, or

- intranasal medications, or

- other prescription medications except the permitted concomitant medications
listed below:

- Permitted concomitant medications (prescription or non-prescription) include
nutritional supplements, medications for gastroesophageal reflux, eye drops, and
topical medications, including (but not limited to) cutaneous (topical) steroids,
topical antibiotics, and topical antifungal agents.

- Any of the following events at the time of enrollment:

- fever (temporal or rectal temperature of greater than or equal to 100.4°F), or

- upper respiratory signs or symptoms (rhinorrhea, cough, or pharyngitis) or

- nasal congestion significant enough to interfere with successful inoculation, or

- otitis media

Inclusion Criteria for RSV-Seronegative Infants and Children:

- Greater than or equal to 6 months of age and less than 25 months of age at the time of
inoculation

- Screening and pre-inoculation serum specimens for RSV-neutralizing antibody are
obtained no more than 42 days prior to inoculation

- Seronegative for RSV antibody, defined as serum RSV-neutralizing antibody titer less
than 1:40

- In good health based on review of the medical record, history, and physical
examination at the time of inoculation

- Received routine immunizations appropriate for age based on the ACIP Recommended
Immunization Schedule for Children and Adolescents Aged 18 Years or Younger

- Growing normally for age as demonstrated on a standard growth chart, AND

- If less than 1 year of age: has a current height and weight above the 5th
percentile for age

- If greater than or equal to 1 year of age: has a current height and weight above
the 3rd percentile for age

- Expected to be available for the duration of the study

- Parent/guardian is willing and able to provide written informed consent

Exclusion Criteria for RSV-Seronegative Infants and Children:

- Born at less than 34 weeks gestation

- Born at less than 37 weeks gestation, and at the date of inoculation less than 1 year
of age

- Maternal history of a positive HIV test

- Evidence of chronic disease

- Known or suspected infection or impairment of immunological functions

- Bone marrow/solid organ transplant recipient

- Major congenital malformations, including congenital cleft palate or cytogenetic
abnormalities

- Suspected or documented developmental disorder, delay, or other developmental problem

- Cardiac abnormality requiring treatment

- Lung disease or reactive airway disease

- More than one episode of wheezing in the first year of life

- Wheezing episode or received bronchodilator therapy within the past 12 months

- Wheezing episode or received bronchodilator therapy after the age of 12 months

- Previous receipt of supplemental oxygen therapy in a home setting

- Previous receipt of an investigational RSV vaccine

- Previous receipt or planned administration of anti-RSV antibody product including
ribavirin, RSV Ig, or RSV mAb

- Previous receipt of immunoglobulin or any antibody products within the past 6 months

- Previous receipt of any blood products within the past 6 months

- Previous anaphylactic reaction

- Previous vaccine-associated adverse reaction that was Grade 3 or above

- Known hypersensitivity to any study product component

- Member of a household that contains an infant who is less than 6 months of age at the
date of inoculation through the 28th day after inoculation

- Member of a household that, at the date of inoculation through the 28th day after
inoculation, contains an immunocompromised individual including but not limited to:

- a person who is HIV-infected

- a person who has received chemotherapy within the 12 months prior to enrollment

- a person receiving immunosuppressant agents

- a person living with a solid organ or bone marrow transplant

- Attends a daycare facility that does not separate children by age and contains an
infant less than 6 months of age at the date of inoculation through the 28th day after
inoculation

- Receipt of any of the following prior to enrollment:

- any inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days
prior, or

- any live vaccine, other than rotavirus vaccine, within the 28 days prior, or

- another investigational vaccine or investigational drug within 28 days prior, or

- salicylate (aspirin) or salicylate-containing products within the past 28 days

- Scheduled administration of any of the following after planned inoculation

- inactivated vaccine or live-attenuated rotavirus vaccine within the 14 days
after, or

- any live vaccine other than rotavirus in the 28 days after, or

- another investigational vaccine or investigational drug in the 56 days after

- Receipt of any of the following medications within 3 days of study enrollment:

- systemic antibacterial, antiviral, antifungal, anti-parasitic, or antituberculous
agents, whether for treatment or prophylaxis, or

- intranasal medications, or

- other prescription medications except the permitted concomitant medications
listed below:

- Permitted concomitant medications (prescription or non-prescription) include
nutritional supplements, medications for gastroesophageal reflux, eye drops, and
topical medications, including (but not limited to) cutaneous (topical) steroids,
topical antibiotics, and topical antifungal agents.

- Any of the following events at the time of enrollment:

- fever (temporal or rectal temperature of greater than or equal to 100.4°F), or

- upper respiratory signs or symptoms (rhinorrhea, cough, or pharyngitis) or

- nasal congestion significant enough to interfere with successful inoculation, or

- otitis media

Inclusion Criteria for RSV-Seronegative Children Participating in a Second Season of RSV
Surveillance:

- RSV-seronegative participants who have completed the initial RSV season surveillance
portion of the study and whose parent/guardian is willing and able to provide written
informed consent

- Expected to be available during the second year of RSV surveillance

Exclusion Criteria for RSV-Seronegative Children Participating in a Second Season of RSV
Surveillance:

- Currently enrolled in another RSV study