RADVAX™: A Trial of Combined Pembrolizumab and Hypofractionated Radiation in Patients With Advanced Urothelial Cancer Who Have Progressed on Anti-PD-1/PD-L1 Monotherapy

Inclusion Criteria:

- Be willing and able to provide written informed consent/assent for the trial.

- Be 18 years of age or older on day of signing informed consent.

- Histologically confirmed diagnosis of pure or mixed urothelial cancer.

- Stage IV cancer by AJCC staging criteria. Patients who are stage IV as a result of
cT4b disease only are not eligible.

- Progression of disease (radiographic or clinical) while on anti-PD-1 or anti-PD-L1
therapy at least 9 weeks or more from the date of starting anti-PD-1 or anti-PD-L1
therapy.

- Presence of an index lesion greater than or equal to 1 cm amenable to hypofractionated
radiotherapy

- Patients who have metastatic cancer must have at least one lesion that is outside the
radiation field that measures greater than one cm that can be followed by RECIST 1.1.
This lesion, if it is close to the radiated lesion, must receive no more than 10% of
the dose prescribed to the target lesion.

- Have provided tissue from an archival tissue sample or newly obtained core or
excisional biopsy of a tumor lesion.

- Have a performance status of 0 or 1 on the ECOG Performance Scale.

- Ability to tolerate hypofractionated radiation therapy (e.g. lie flat and hold
position)

- Demonstrate adequate organ function, all screening labs should be performed within 14
days of treatment initiation.

- Patients on dialysis are excluded from this study.

- Female subject of childbearing potential should have a negative urine or serum
pregnancy within 72 hours prior to receiving the first dose of study medication. If
the urine test is positive or cannot be confirmed as negative, a serum pregnancy test
will be required.

- Female subjects of childbearing potential should be willing to use 2 methods of birth
control or be surgically sterile, or abstain from heterosexual activity for the course
of the study through 120 days after the last dose of study medication. Subjects of
childbearing potential are those who have not been surgically sterilized or have not
been free from menses for more than 1 year. 14. Male subjects should agree to use an
adequate method of contraception starting with the first dose of study therapy through
120 days after the last dose of study therapy.

Exclusion Criteria

- Is currently participating in or has participated in a study of an investigational
agent or using an investigational device within 4 weeks of the first dose of
treatment.

- Has a diagnosis of immunodeficiency or is receiving systemic steroid therapy or any
other form of immunosuppressive therapy within 7 days prior to the first dose of trial
treatment. Systemic steroids administered specifically as a premedication for
chemotherapy infusion or radiotherapy are allowed.

- Has had a prior monoclonal antibody within 4 weeks prior to study Day 1 or who has not
recovered (i.e., less than or at Grade 1 or at baseline) from adverse events due to
agents administered more than 4 weeks earlier.

- Has had prior chemotherapy, targeted small molecule therapy, or radiation therapy
within 2 weeks prior to study Day 1 or who has not recovered (i.e., less than or at
Grade 1 or at baseline) from adverse events due to a previously administered agent. -
Note: Subjects with less than or Grade 2 neuropathy are an exception to this criterion
and may qualify for the study. - Note: If subject received major surgery, they must
have recovered adequately from the toxicity and/or complications from the intervention
prior to starting therapy.

- A history of prior radiotherapy that precludes delivery of hypofractionated
radiotherapy

- Has a known additional malignancy that is progressing or requires active treatment.

Exceptions include basal cell carcinoma of the skin, squamous cell carcinoma of the skin,
or in situ cervical cancer that has undergone potentially curative therapy.

- Has known active central nervous system (CNS) metastases and/or carcinomatous
meningitis. Subjects with previously treated brain metastases may participate provided
they are stable (without evidence of progression by imaging for at least four weeks
prior to the first dose of trial treatment and any neurologic symptoms have returned
to baseline), have no evidence of new or enlarging brain metastases, and are not using
steroids for at least 7 days prior to trial treatment.

- Has an active autoimmune disease requiring systemic treatment within the past 3 months
or a documented history of clinically severe autoimmune disease, or a syndrome that
requires systemic steroids or immunosuppressive agents. Subjects with vitiligo or
resolved childhood asthma/atopy would be an exception to this rule. Subjects that
require intermittent use of bronchodilators or local steroid injections would not be
excluded from the study. Subjects with hypothyroidism stable on hormone replacement or
Sjogrens syndrome will not be excluded from the study.

- Has evidence of interstitial lung disease or active, non-infectious pneumonitis.

- Has an active infection requiring systemic therapy.

- Has a history or current evidence of any condition, therapy, or laboratory abnormality
that might confound the results of the trial, interfere with the subjects
participation for the full duration of the trial, or is not in the best interest of
the subject to participate, in the opinion of the treating investigator.

- Has known psychiatric or substance abuse disorders that would interfere with
cooperation with the requirements of the trial.

- Is pregnant or breastfeeding, or expecting to conceive or father children within the
projected duration of the trial, starting with the prescreening or screening visit
through 120 days after the last dose of trial treatment.

- Has a known history of Human Immunodeficiency Virus (HIV) (HIV 1/2 antibodies).

- Has known active Hepatitis B (e.g., HBsAg reactive) or Hepatitis C (e.g., HCV RNA
[qualitative] is detected).

- Has received a live vaccine within 30 days prior to the first dose of trial treatment.

- Had progression of disease within 9 weeks of starting prior anti-PD-1 or anti-PD-L1
monotherapy therapy.

- Has not had prior cisplatinum-based chemotherapy, unless the subject is considered
cisplatinum-ineligible based on meeting at least one of the following criteria: a.
ECOG performance status of 2 (the proportion of ECOG 2 subjects will be limited to
approximately 50% of the total population) b. Creatinine clearance (calculated or
measured) less than 60 mL/min but greater than 30 mL/min Note: Subjects with a
creatinine clearance (calculated or measured) less than 30 mL/min or on dialysis are
excluded from the trial. c. CTCAE v.4, Greater than Grade 2 audiometric hearing loss
(25dB in two consecutive wave ranges) d. CTCAE v.4, Greater than Grade 2 peripheral
neuropathy e. NYHA Class III heart failure