Vaccine Therapy in Treating Patients With Stage IV HLA-A2 and HER2 Positive Breast or Ovarian Cancer Receiving Trastuzumab

PRIMARY OBJECTIVES:

I. To evaluate the safety of administering a HER2 cytotoxic T-cell (CTL) peptide-based
vaccine (HER-2/neu peptide vaccine) to stage IV breast and ovarian cancer patients receiving
maintenance trastuzumab.

II. To quantify and characterize antigen specific T cell subsets specific to HER2 in
peripheral blood mononuclear cell (PBMC) of patients after vaccination with a HER2 CTL
peptide-based vaccine while receiving maintenance trastuzumab.

SECONDARY OBJECTIVES:

I. To evaluate overall survival (OS) in patients who complete a vaccination series with a
HER2 CTL peptide-based vaccine while receiving maintenance trastuzumab.

OUTLINE:

Patients receive HER-2/neu peptide vaccine intradermally (ID) once per month for 6 months in
the absence of disease progression or unacceptable toxicity.

After completion of study treatment, patients are followed up at 1, 3, 6, and 12 months, and
then yearly for up to 5 years.

Inclusion Criteria:

- Subjects must have either stage IV breast or ovarian cancer in remission or with
stable disease on trastuzumab monotherapy

- HER2 overexpression by immunohistocytochemistry (IHC) of 2+ or 3+, in the primary
tumor or metastasis; if overexpression is 2+ by IHC, then patients must have HER2 gene
amplification documented by fluorescence in situ hybridization (FISH)

- Subjects must be HLA-A2 positive

- Eligible subjects must have completed appropriate treatment for their primary disease
and be off cytotoxic chemotherapy and any immunosuppressive agents such as systemic
steroids for at least 30 days prior to enrollment; patients should continue
trastuzumab monotherapy throughout the course of this protocol; concurrent hormonal
and biphosphonate therapies are allowed

- Subjects must have an Eastern Cooperative Oncology Group (ECOG) performance status
score = 0 or 1

- Male subjects must agree to contraceptive use during the study period (7 months) and
non-menopausal female subjects must agree to contraception for the remainder of their
childbearing years

- Hematocrit >= 30 performed within 60 days of enrollment

- Platelet count >= 100,000 performed within 60 days of enrollment

- White blood cells (WBC) >= 3000/ul performed within 60 days of enrollment

- Stable creatinine =< 2.0 mg/dL or creatinine clearance >= 60 ml/min performed within
60 days of enrollment

- Serum bilirubin < 1.5 mg/dl performed within 60 days of enrollment

- Serum glutamic-oxaloacetic transaminase (SGOT) < 2 x upper limit of normal (ULN)
performed within 60 days of enrollment

- Subjects must have recovered from major infections and/or surgical procedures and, in
the opinion of the investigator, not have a significant active concurrent medical
illness precluding protocol treatment or survival

- Patients must have a baseline left ventricular ejection fraction (LVEF) measured by
multi-gated acquisition scan (MUGA) equal to or greater than the lower limit of normal
for the radiology facility and if there are two consecutive MUGAS performed while on
trastuzumab from the same radiology facility, there cannot be a decrease in LVEF of >
15% from the original MUGA scan

Exclusion Criteria:

- Subjects cannot be simultaneously enrolled on other treatment studies

- Any contraindication to receiving granulocyte-macrophage colony-stimulating factor
(GM-CSF) based vaccine products

- Cardiac disease, specifically restrictive cardiomyopathy, unstable angina within the
last 6 months prior to enrollment, New York Heart Association functional class III-IV
heart failure on active treatment with normalized LVEF on therapy, and symptomatic
pericardial effusion

- Active autoimmune disease

- Subjects cannot have an active immunodeficiency disorder, e.g. human immunodeficiency
virus (HIV)