Effect of Exercise on Genes That Control Muscle Function
| Status: | Recruiting |
|---|---|
| Conditions: | Healthy Studies |
| Therapuetic Areas: | Other |
| Healthy: | No |
| Age Range: | 20 - 40 |
| Updated: | 7/19/2018 |
| Start Date: | February 1, 2018 |
| End Date: | December 31, 2022 |
| Contact: | Dennis Villareal, MD |
| Email: | dennis.villareal@bcm.edu |
| Phone: | 713-794-7156 |
Epigenomic Remodeling of Metabolism by Exercise
The proposed project will examine how exercise counteracts metabolic disorders and type 2
diabetes through regulating gene expression. The project is highly relevant to public health
because of the global pandemic of diabetes, obesity, and associated metabolic syndromes as
well as the well-known metabolic benefit of physical exercise in correcting these disorders.
diabetes through regulating gene expression. The project is highly relevant to public health
because of the global pandemic of diabetes, obesity, and associated metabolic syndromes as
well as the well-known metabolic benefit of physical exercise in correcting these disorders.
Exercise is a first-line treatment for type 2 diabetes, and exerts its beneficial effects not
only by burning off energy but also by causing prolonged metabolic changes through changing
gene expression. Genes are our genetic materials and the expression of genes determines our
biology. In our previous study in animals, we identified some factors that drive
exercise-induced gene expression changes. Here we would like to address whether the result is
also true in human. This work will provide molecular insights into how exercise remodels our
metabolism and will potentially find a way to maximize the benefit we get from physical
exercise.
The purpose of this study is to determine whether acute exercise activate certain molecular
factors in human skeletal muscle. Participants will be asked to undergo an acute bout of
aerobic exercise at ~ moderate intensity for about 2 hours. Before and after the exercise,
the participants will undergo a muscle biopsy. The muscle tissues will be used for total RNA
extraction and RT-qPCR analysis of genes that include but are not limited to de facto
JunD/AP-1 target genes and will also be analyzed by Jun D Chip-qPCT to assess binding of Jun
D on its de facto target genes.
only by burning off energy but also by causing prolonged metabolic changes through changing
gene expression. Genes are our genetic materials and the expression of genes determines our
biology. In our previous study in animals, we identified some factors that drive
exercise-induced gene expression changes. Here we would like to address whether the result is
also true in human. This work will provide molecular insights into how exercise remodels our
metabolism and will potentially find a way to maximize the benefit we get from physical
exercise.
The purpose of this study is to determine whether acute exercise activate certain molecular
factors in human skeletal muscle. Participants will be asked to undergo an acute bout of
aerobic exercise at ~ moderate intensity for about 2 hours. Before and after the exercise,
the participants will undergo a muscle biopsy. The muscle tissues will be used for total RNA
extraction and RT-qPCR analysis of genes that include but are not limited to de facto
JunD/AP-1 target genes and will also be analyzed by Jun D Chip-qPCT to assess binding of Jun
D on its de facto target genes.
Inclusion Criteria:
- Age 20 - 40 years
- BMI 18.5 - 29.9
- Stable body weight (not more than 2 kg change) during the past 6 months
- Moderate sedentary (regular exercise less than 1 hour per week for the last 6 months)
- Willing to provide informed consent
Exclusion Criteria:
- Failure to provide informed consent
- Any major chronic disease or any condition that would interfere with exercise, in
which exercise is contraindicated, or that would interfere with interpretation of
results
- Severe orthopedic and or neuromuscular disease that would contraindicate participation
in exercise
- Other significant co-morbid disease that would impair ability to exercise
- Uncontrolled hypertension (BP greater than 160/90)
- History of malignancy during the past 5 years
- Diabetes mellitus as determined by self-report with verification (medical records,
current treatment, confirmation from health care provider), or HbA1c of exceeding 6.5%
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