SGI-110 Plus Durvalumab/Tremelimumab in SCLC

SCLC accounts for approximately 15% of new cases of lung cancer, and an estimated 33,000
cases are expected to be diagnosed in the United States in 2016. Compared to NSCLC, SCLC
typically has a more rapid doubling time, a higher growth fraction, and earlier development
of distant metastases. Patients with limited stage (LS) disease are treated with curative
intent using definitive, concurrent chemotherapy and thoracic radiotherapy. For patients with
extensive stage (ES) disease, systemic chemotherapy can prolong survival in most cases,
however long-term survival is rare. Despite the activity of several agents in SCLC, an
etoposide plus platinum (i.e. cisplatin) doublet regimen remains the standard of care in the
first-line setting because of its higher activity compared to other chemotherapy regimens, as
well as the ease of combining it with radiation. Initial response rates may be as high as
70-90% in LS-SCLC and 50-70% in ES-SCLC. However, the disease typically recurs rapidly which
is reflected by median survival rates of 9 to 11 months for ES-SCLC and a 2-year survival
rate of less than 5%.

This study has a 3 + 3 design that will be used to assess the safety of SGI-110 given prior
to flat doses of durvalumab (1500 mg) and tremelimumab (75 mg). The starting dose of SGI-110
will be 30 mg/m2 (dose level 0) and the target dose that is predicted to be safe and most
effective will be 45 mg/m2 (dose level 1). These doses have been chosen based on safety and
efficacy data from phase 1 clinical trials in other solid tumors, as described above.
Patients enrolled in any given dose level will be evaluated for safety (adverse events
monitoring) and efficacy. There will be mandatory pre- and on-treatment tumor biopsies
performed in alternating fashion on cycle 1 day 8 +/- 2 days or cycle 2 day 8 +/- 2 days. The
dose-limiting toxicity (DLT) observation period will last for 4 weeks (28 days) and ends on
C2D1. Delayed serious immune-mediated adverse events will also be monitored but will not be
considered dose limiting toxicities.

Inclusion Criteria:

For inclusion in the study, patients should fulfill the following criteria:

1. Written informed consent and any locally-required authorization (e.g., HIPAA in the
USA, EU Data Privacy Directive in the EU) obtained from the subject prior to
performing any protocol-related procedures, including screening evaluations

2. Age ≥ 18 years at time of study entry

3. Eastern Cooperative Oncology Group (ECOG performance status of 0 or 1)

4. Life expectancy of ≥ 12 weeks

5. Adequate normal organ and marrow function as defined below Hemoglobin ≥ 9.0 g/dL
Absolute neutrophil count (ANC) ≥ 1.5 x 109/L (> 1500 per mm3) Platelet count ≥ 100 x
109/L (>100,000 per mm3) Serum total bilirubin ≤ 1.5 x institutional upper limit of
normal (ULN) (for patients with a diagnosis of Gilbert's syndrome, direct bilirubin ≤
1.5 x ULN) AST (SGOT)/ALT (SGPT) ≤ 2.5 x institutional upper limit of normal unless
liver metastases are present, in which case it must be ≤ 5x ULN

Serum creatinine CL>40 mL/min by the Cockcroft-Gault formula (Cockcroft and Gault
1976) or by 24-hour urine collection for determination of creatinine clearance:

Males:

Creatinine CL (mL/min) = Weight (kg) x (140 - Age) . 72 x serum creatinine (mg/dL)

Females:

Creatinine CL (mL/min) = Weight (kg) x (140 - Age) x 0.85 72 x serum creatinine
(mg/dL)

6. Females of childbearing potential who are sexually active with a non-sterilized male
partner must use a highly effective method of contraception from the time of
screening, and must agree to continue using such precautions for 180 days after the
final dose of investigational product. Cessation of contraception after this point
should be discussed with a responsible physician. Periodic abstinence, the rhythm
method, and the withdrawal method are not acceptable methods of contraception. Female
subjects must also refrain from egg cell donation for 180 days after the final dose of
investigational product.

A) Females of childbearing potential are defined as those who are not surgically
sterile (i.e. bilateral tubal ligation, bilateral oophorectomy, or complete
hysterectomy) or those who are not postmenopausal (defined as 12 months with no menses
with postmenopausal gonadotropin levels [luteinizing hormone and follicle-stimulating
hormone], or estradiol levels within the postmenopausal range according to local
guidelines without an alternative medical cause).

B) A highly effective method of contraception is defined as one that results in a low
failure rate (i.e. less than 1% per year) when used consistently and correctly.

7. Subject is willing and able to comply with the protocol for the duration of the study
including undergoing treatment and scheduled visits and examinations including follow
up.

8. Subjects must have a histologically confirmed diagnosis of small cell lung carcinoma.
A fresh, pre-treatment tumor biopsy will be required to evaluate tumor infiltrating
lymphocytes, PD-L1 IHC staining, methylation status, etc. as outlined in the study
timeline. All subjects are also required to have a C1D8 (or C2D8) biopsy.

9. Subjects must have extensive-stage disease (by NCCN criteria) that is progressive or
relapsed after platinum-based chemotherapy.

10. Tumor burden must be radiographically measurable by RECIST criteria.

11. At time of Day 1 of the study, subjects with central nervous system metastases must
have been treated and must be asymptomatic and meet the following:

1. No concurrent treatment, inclusive of but not limited to surgery, radiation,
and/or corticosteroids

2. Neurologic stability (lack of signs and symptoms greater than baseline prior to
XRT) until the time of dosing

3. For radiation treatment, there should be at least 14 days between the last day of
stereotactic radiosurgery or gamma-knife treatment and Day 1 of protocol
treatment. For WBRT, there should be at least 28 days between last day of WBRT
and Day 1 of protocol treatment.

4. Note: patients with leptomeningeal disease or cord compression are excluded from
the study.

Exclusion Criteria:

Subjects should not enter the study if any of the following exclusion criteria are
fulfilled:

1. Involvement in the planning and/or conduct of the study (applies to both AstraZeneca
staff and/or staff at the study site).

2. Previous enrollment in the present study.

3. Participation in another clinical study with an investigational product during the
last 4 weeks.

4. Any previous treatment with a PD1 or PD-L1 inhibitor, including durvalumab or an
anti-CTLA4, including tremelimumab

5. Any previous treatment with a hypomethylating agent, including decitabine,
azacitidine, or SGI-110.

6. History of another primary malignancy except for:

- Malignancy treated with curative intent and with no known active disease ≥ 5
years before the first dose of study drug and of low potential risk for
recurrence

- Adequately treated non-melanoma skin cancer or lentigo maligna without evidence
of disease

- Adequately treated carcinoma in situ without evidence of disease e.g., cervical
cancer in situ

7. Receipt of the last dose of anti-cancer therapy (chemotherapy, immunotherapy,
endocrine therapy, targeted therapy, biologic therapy, tumor embolization, monoclonal
antibodies, gamma-knife, other investigational agent) ≤ 14 days prior to the first
dose of study drug. For WBRT, the washout period is 28 days. Local treatment of
isolated lesions for palliative RT (by radiotherapy, for example) is acceptable.

8. Mean QT interval corrected for heart rate (QTc) ≥ 470 ms calculated from
electrocardiogram (in triplicate, if applicable) using Fredericia's Correction

9. Liver cirrhosis or chronic liver disease Childs-Pugh B or C.

10. Current or prior use of immunosuppressive medication within 28 days before the first
dose of durvalumab or tremelimumab, with the exceptions of intranasal and inhaled
corticosteroids or systemic corticosteroids at physiological doses, which are not to
exceed 10 mg/day of prednisone, or an equivalent corticosteroid.

11. Receipt of the tyrosine kinase inhibitor sunitinib within 90 days before the first
dose of study therapy.

12. Any unresolved toxicity ( > CTCAE grade 2) from previous anti-cancer therapy.

13. Any prior Grade ≥ 3 immune-related adverse event (irAE) while receiving any previous
immunotherapy agent, or any unresolved irAE >Grade 1

14. Active or prior documented autoimmune disease within the past 2 years NOTE: Subjects
with vitiligo, Grave's disease, or psoriasis not requiring systemic treatment (within
the past 2 years) are not excluded.

15. Active or prior documented history of pneumonitis or interstitial lung disease.

16. Active or prior documented inflammatory bowel disease (e.g., Crohn's disease,
ulcerative colitis)

17. History of primary immunodeficiency

18. History of allogeneic organ transplant

19. History of hypersensitivity to durvalumab, tremelimumab, SGI-110, or any excipient.

20. Uncontrolled intercurrent illness including, but not limited to, ongoing or active
infection, symptomatic congestive heart failure, uncontrolled hypertension, unstable
angina pectoris, cardiac arrhythmia, active peptic ulcer disease or gastritis, active
bleeding diatheses including any subject known to have evidence of acute or chronic
hepatitis B, hepatitis C or human immunodeficiency virus (HIV), or psychiatric
illness/social situations that would limit compliance with study requirements or
compromise the ability of the subject to give written informed consent

21. Known history of active tuberculosis

22. Leptomeningeal carcinomatosis or cord compression

23. Receipt of live attenuated vaccination within 30 days prior to study entry or within
30 days of receiving durvalumab or tremelimumab

24. Female subjects who are pregnant or breast-feeding, or male or female patients of
reproductive potential who are not willing to employ a highly effective method of
contraception from screening to 180 days after the last dose of investigational
therapy.

25. Any condition that, in the opinion of the investigator, would interfere with
evaluation of study treatment or interpretation of patient safety or study results

26. Symptomatic or uncontrolled brain metastases requiring concurrent treatment (surgery,
RT, corticosteroids)

27. Subjects with uncontrolled seizures.

28. Concomitant use of drugs with laxative properties and/or herbal/natural remedies for
constipation. These agents should be avoided for 90 days after the last dose of
investigational therapy, given the potential for exacerbation of diarrhea.

29. Known significant mental illness or other conditions such as active alcohol or other
substance abuse/addiction that, in the opinion of the investigator, predisposes the
subject to high risk of noncompliance with the protocol.

Procedures for withdrawal of incorrectly enrolled patients are presented in Section 5.5.1.
If a patient withdraws from participation in the study, then his or her
enrollment/randomization code cannot be reused. Withdrawn patients will not be replaced.