Hypoglycemia and Autonomic Nervous System Function- B2

Participants are admitted for two days and two nights to an inpatient clinical research
center for performance of study procedures. On Day 1, two hyperinsulinemic hypoglycemic (50
mg/dl) clamps are performed - an AM clamp from about 9 to 11 am and a PM clamp from about 1
to 3 pm.. Modified Oxford procedures are performed in duplicate at five time points -- before
the AM clamp, during the AM clamp, before the PM clamp, during the PM clamp, and the morning
of Day 2 (about 16 hrs after completion of the the PM clamp). The Modified Oxford procedure
involves the sequential intravenous administration of nitroprusside and phenylephrine with
assessment of blood pressure and heart rate by finapress. Baroreflex sensitivity is the
relationship of changes in heart rate to changes in blood pressure. .

Inclusion Criteria:

- Males and females age 18 to 55 years

- Type 2 Diabetes Mellitus controlled by diet, exercise and non-insulin glycemic control
agents (metformin, thiazolidinediones, sulfonylureas, dipeptidyl peptidase-4
inhibitors, sodium-glucose co-transporter 2 inhibitors, alpha-glucosidase inhibitors
or glucagon-like peptide-1 receptor agonist) or basal insulin at doses less than or
equal to 0.3 units/ kg per day.

Exclusion Criteria:

- Hemoglobin A1c > 9% for type 2 diabetes

- Recurrent hypoglycemic episodes within the past month

- BMI < 25 or > 42 (Type 2 diabetes only)

- Pregnancy

- Lactation

- Clinically significant coronary artery, cerebrovascular, or peripheral vascular
disease, or presence of systemic illness that might affect autonomic function. Such
illnesses include congestive heart failure, renal, pulmonary, hepatic disease, anemia,
malignancies, untreated thyroid disease, and alcoholism.

- Current major depressive illness

- In all subjects, any individuals on oral, injected, inhaled or topical corticosteroids
within the last year or oral contraceptives within the past 3 months will be excluded.

- Known hypersensitivity to nitroglycerin, nitroprusside and/or phenylephrine.

- Blood pressure > 160/100 mmHg (applies to participants with T2 diabetes)

- Creatinine > 1.5 mg/dL

- Serum potassium >5.2 mmol/L

- Estimated GFR < 50 mL/min

- Use of Viagra, Cialis, and similar drugs within 72 hours of admission.

- Use of beta-blockers or mineralocorticoid receptor antagonists