Pharmacokinetic Drug Interactions of AEGR-733 on Lipid-lowering Agents

Objectives:

Primary: To evaluate the effects of low and high doses of AEGR-733 on the pharmacokinetics of
6 FDA-approved medications that are likely to be used in combination with AEGR-733 as
assessed by:

• Pharmacokinetic parameters: Cmax, Tmax, T1/2, and AUC (area under the curve).

Secondary: To evaluate the safety of AEGR-733 in combination with other lipid lowering agents
in healthy subject as assessed by:

- Changes in associated liver enzymes AST, ALT and, Alkaline Phosphatase, & Total
Bilirubin.

- Changes in all reported adverse events.

- To evaluate the effects of AEGR-733 in combination with other lipid lowering agents on
the following lipids and lipoproteins: TC, LDL-C, VLDL, TG, HDL-C, ApoB and ApoAI.

4.0 STUDY DESIGN AND RATIONALE

4.1 STUDY DESIGN This is a single-center, phase II, clinical trial consisting of a eight (8)
day open-label phase to assess the pharmacokinetic drug interactions of AEGR-733 on 6 probe
drugs in healthy volunteers, followed by a one week safety visit. 105 subjects will be
enrolled into this fixed-sequenced research study. Eligible subjects based on the screening
visit will come to the GCRC for an inpatient visit (25-36 hr depending on if they come in
evening before study day 1 or morning of). On the morning of study day 1, subjects will be
assigned to one of 6 probe drugs(A-H below) and will take one dose of this medication. Timed
blood samples will be drawn just before the administration of the probe drug and during the
following times after drug administration (1,2,3,4,5,6,8,10,12,18, and 24 hrs). Prior to
discharge after the 24 h blood sample, subjects will take an oral dose of AEGR-733 at 10 mg
or 60 mg. Subjects will be given a 5 day supply of AEGR-733 at 10 mg or 60 mg to be taken
once daily in the morning for the next 5 days (through day 7). On study day 8, subjects will
take a final dose of AEGR-733 at 10 mg or 60 mg (total doses= 7) simultaneously with the same
probe drug they took on day 1. Timed blood samples will be drawn just before the
administration of the probe drug and AEGR-733 as well as 1,2,3,4,5,6,8,10,12,18, and 24 hours
after study drug administration. After the 24 hour blood sample, subjects will be discharged.
15 subjects who participate in this study will receive dextromethorphan as the probe drug,
which requires urine collection for 8 hours post dose. Blood for pharmacokinetic samples will
not be collected on these subjects. Subjects receiving dextromethorphan may leave after the 8
hour urine collection at visits 2 and 3 (referred to as the inpatient visits). All subjects
will come back 1 week later for a final visit to check safety lab parameters including liver
transaminases and total bilirubin. Subjects will be instructed to abstain from drinking any
alcoholic beverages once screened until study completion. Subjects who are not willing to
comply with these requests will not be enrolled.

The FDA-approved lipid-lowering therapies will include:

A) Atorvastatin, 20 mg (n=15)and AEGR-733 10 mg B) Ezetimibe, 10 mg (n=10)and AEGR-733 10 mg
C) Simvastatin, 20 mg (n=15)and AEGR-733 10 mg D) Rosuvastatin, 20 mg (n=10)and AEGR-733 10
mg E) Micronized fenofibrate, 145 mg (n=10)and AEGR-733 10 mg F) Atorvastatin, 20 mg (n=15)
and AEGR-733 60 mg G) Rosuvastatin, 20 mg (n=15) and AEGR-733 60 mg H) Dextromethorphan, 30
mg (n=15) and AEGR-733 60 mg I) Extended Release Niacin, 1000 mg (n=20) and AEGR-733 10 mg

Inclusion Criteria:

Males and non-pregnant/non-lactating female subjects between the ages of 18 and 70 who are
in good overall health.

To be eligible for enrollment in this study, patients must meet all of the following
criteria:

1. Men and women between the ages of 18 and 70

2. Women of child-bearing potential, that is, women not surgically sterilized and between
menarche and 1 year post menopause, must test negative for pregnancy at the time of
enrollment based on a urine pregnancy test and agree to use a reliable method of
non-medication birth control (for example, a reliable barrier method of birth control
[diaphragms with contraceptive jelly; cervical caps with contraceptive jelly; condoms
with contraceptive foam; intrauterine devices]; partner with vasectomy; or abstinence)
during the study and for one month following the last dose of study drug.

3. Subjects must be in good overall health

4. Subjects must be able to comprehend and willing to provide a signed IRB approved
Informed Consent Form.

5. Subjects must be willing to comply with all study-related procedures.

Exclusion Criteria:

1. Known atherosclerotic cardiovascular disease, including coronary disease,
cerebrovascular disease, or peripheral vascular disease

2. History of diabetes mellitus or fasting glucose > 126 mg/dL at the screening visit.

3. History of a non-skin malignancy within the previous 5 years

4. Renal insufficiency as defined by creatinine > 1.3 mg/dl

5. Any major active rheumatologic, pulmonary, or dermatologic disease or inflammatory
condition

6. History of hypertension

7. Known coagulopathy and /or elevated PT/PTT >1.5 x ULN

8. Oral history of HIV positive

9. Patients who have undergone any organ transplant

10. Known active fibrotic or cirrhotic disease; ALT or AST > 1.5x ULN

11. Any major surgery within the previous 3 months

12. Individuals who currently use tobacco products or have done so in the previous 30 days

13. History of drug abuse (< 3 years)

14. Regular use of alcoholic beverages (> 7 drinks/day)

15. Subjects who do not agree to abstain from consuming alcoholic beverages during the
entire study duration.

16. Body mass index (BMI) > 30 kg/m2 or < 18.5 kg/m2

17. Participation in an investigational drug study within 6 weeks prior to the screening
visit

18. Serious or unstable medical or psychological conditions that, in the opinion of the
investigator, would compromise the subject's safety or successful participation in the
study will be excluded.

19. Currently taking any prescription, including oral contraceptives, or OTC medication
regularly that cannot be stopped for at least 30 days prior to enrollment until
completion of the study

20. Regular consumers of grapefruit juice, or have taken any medications known to be
metabolized by CYP 3A4 within 4 weeks prior to the screening visit (ie. SSRIs,
anti-fungals, anti-biotics, etc)

21. History of myalgia with a statin or unknown hypersensitivity to any statin, zetia,
AEGR-733, or fenofibrate.