The Effect of Time-restricted Feeding on Physiological Function in Middle-aged and Older Adults

Overall, the proposed research project has the long-term potential to influence clinical
practice by establishing novel therapies for treating multiple domains of age-associated
physiological dysfunction and thereby reducing the risk of clinical disease and disability.

Inclusion Criteria:

- Ability to provide informed consent

- Baseline brachial flow-mediated dilation (FMD) < 6%Δ (rationale: non-invasive
screening to ensure exclusion of subjects with exceptionally high baseline endothelial
function.

- Ability to perform motor and cognitive tests (e.g., can rise from a chair, walk for 2
min, climb 10 stairs)

- Women will be confirmed as postmenopausal (either natural or surgical) based on
cessation of menses for >1 year.

Exclusion Criteria:

- Ages <55 years or ≥80 years

- Occupation that requires night-shift work or abnormal hours that would disrupt eating
schedule

- Dietary habit of regularly skipping meals, already eating within a ~10 hour window, or
any other form of restrictive eating.

- Unable to adhere to an 8 hour eating window for the length of the study period

- Participation in regular vigorous aerobic/endurance exercise (>3 vigorous bouts/week)

- Not weight stable in the prior 3 months (>2 kg weight change).

- Body mass index (BMI) >40 kg/m^2 (rationale: vascular function measurements can be
inaccurate in severely obese subjects)

- Current smoking

- Diagnosis of a chronic clinical disease (e.g., coronary artery/peripheral
artery/cerebrovascular diseases, diabetes, chronic kidney disease requiring dialysis,
neurological disorders or diseases that may affect motor/cognitive functions [multiple
sclerosis, Parkinson's disease, polio, Alzheimer's disease, dementia or other brain
diseases of aging]), except hypertension and hyperlipidemia.

- Having unstable angina, acute myocardial infarction, coronary angioplasty, or aorto-
coronary bypass surgery as defined by the occurrence of an event, symptom, surgery, or
change in medication and/or dosage within 3 months prior to enrollment.

- Having thyroid disease that is not controlled by medications or <3 month's use of a
particular medication and/or dosage (rationale: uncontrolled thyroid diseases are
associated with alterations in vascular function).

- Having past or present alcohol dependence or abuse, as defined by the American
Psychiatry Association, Diagnostic and Statistical Manual of Mental Disorders