Oatmeal Effect on N-acyl-phosphatidylethanoleamines
| Status: | Completed |
|---|---|
| Conditions: | Obesity Weight Loss, Peripheral Vascular Disease |
| Therapuetic Areas: | Cardiology / Vascular Diseases, Endocrinology |
| Healthy: | No |
| Age Range: | 18 - 65 |
| Updated: | 1/12/2019 |
| Start Date: | October 7, 2018 |
| End Date: | December 31, 2018 |
Acute Effects of Dietary Oatmeal on Serum Levels of N-acyl-phosphatidylethanoleamines and Their Metabolites.
N-acyl-phosphatidylethanolamine (NAPEs) and their active metabolites, N-acyl-ethanolamides
(NAEs) are lipid satiety factors that are normally biosynthesized in the intestinal tract in
response to food intake. Reduced levels of NAPEs and NAEs have been found in obese
individuals, and increasing plasma NAPE and NAEs levels may be beneficial to obese
individuals trying to lose weight or to keep off weight gain after losing weight. We have
found that oatmeal has large amounts of NAPEs, and based on previous mouse studies, we
hypothesize that a single dose of dietary oatmeal is sufficient to double plasma NAE from
baseline, possibly inducing satiety and increasing basal metabolic rate. To test this
hypothesis, we will feed volunteers a single weight-based serving of oatmeal while monitoring
its effects on serum glucose, NAPE and NAE levels as well as on subjective satiety.
(NAEs) are lipid satiety factors that are normally biosynthesized in the intestinal tract in
response to food intake. Reduced levels of NAPEs and NAEs have been found in obese
individuals, and increasing plasma NAPE and NAEs levels may be beneficial to obese
individuals trying to lose weight or to keep off weight gain after losing weight. We have
found that oatmeal has large amounts of NAPEs, and based on previous mouse studies, we
hypothesize that a single dose of dietary oatmeal is sufficient to double plasma NAE from
baseline, possibly inducing satiety and increasing basal metabolic rate. To test this
hypothesis, we will feed volunteers a single weight-based serving of oatmeal while monitoring
its effects on serum glucose, NAPE and NAE levels as well as on subjective satiety.
N-acyl-phosphatidylethanolamine (NAPEs) and their active metabolites, N-acyl-ethanolamides
(NAEs) are lipid satiety factors that are normally biosynthesized in the intestinal tract in
response to food intake. Reduced levels of NAPEs and NAEs have been found in obese
individuals, so that increasing plasma NAPE and NAEs levels may be beneficial to obese
individuals trying to lose weight or to keep off weight gain after losing weight. While NAPEs
are endogenously synthesized by mammals in their intestinal tract, many other organisms,
including plants, also biosynthesize NAPEs. Recent screening of a wide range of foods by our
lab demonstrated that oatmeal (Regular Instant Oatmeal) had very large amounts of NAPEs (0.17
mg NAPE / g dry oatmeal), suggesting that consumption of oatmeal may be a straightforward
mechanism for elevating plasma NAPE and NAE levels in obese individuals. Based on our
previous mouse studies with synthetic NAPE and with bacteria biosynthesizing NAPE, we
hypothesize that a dose of oatmeal sufficient to deliver 0.135 mg NAPE per kg body should be
sufficient to double plasma NAE levels from baseline, thereby inducing satiety and increasing
basal metabolic rate. For a 100 kg person, 80 grams of dry regular instant oatmeal (2
servings) provides this 0.135 mg / kg dose. (The dose of dry oatmeal per person = (body
weight in kg / 100 kg)* 80 g dry oatmeal. Thus for 50 kg person, 40 g dry oatmeal (1 serving)
provides the required dose.) This amount of dry oatmeal is converted to the test meal by
adding sufficient water to overtop oatmeal by about 1 cm (or to consistency desired by
volunteer) and then microwaving for ~3-4 minutes on high. In the initial testing, no
additives such as butter, cream, or brown sugar should be used in preparation or serving
oatmeal. Water can be consumed ad lib.
(NAEs) are lipid satiety factors that are normally biosynthesized in the intestinal tract in
response to food intake. Reduced levels of NAPEs and NAEs have been found in obese
individuals, so that increasing plasma NAPE and NAEs levels may be beneficial to obese
individuals trying to lose weight or to keep off weight gain after losing weight. While NAPEs
are endogenously synthesized by mammals in their intestinal tract, many other organisms,
including plants, also biosynthesize NAPEs. Recent screening of a wide range of foods by our
lab demonstrated that oatmeal (Regular Instant Oatmeal) had very large amounts of NAPEs (0.17
mg NAPE / g dry oatmeal), suggesting that consumption of oatmeal may be a straightforward
mechanism for elevating plasma NAPE and NAE levels in obese individuals. Based on our
previous mouse studies with synthetic NAPE and with bacteria biosynthesizing NAPE, we
hypothesize that a dose of oatmeal sufficient to deliver 0.135 mg NAPE per kg body should be
sufficient to double plasma NAE levels from baseline, thereby inducing satiety and increasing
basal metabolic rate. For a 100 kg person, 80 grams of dry regular instant oatmeal (2
servings) provides this 0.135 mg / kg dose. (The dose of dry oatmeal per person = (body
weight in kg / 100 kg)* 80 g dry oatmeal. Thus for 50 kg person, 40 g dry oatmeal (1 serving)
provides the required dose.) This amount of dry oatmeal is converted to the test meal by
adding sufficient water to overtop oatmeal by about 1 cm (or to consistency desired by
volunteer) and then microwaving for ~3-4 minutes on high. In the initial testing, no
additives such as butter, cream, or brown sugar should be used in preparation or serving
oatmeal. Water can be consumed ad lib.
Inclusion Criteria:
- Adult
- non-pregnant
- normal BMI (20-25)
Exclusion Criteria:
- Obese
- underweight
- diabetes mellitus
- coronary artery disease
- oatmeal allergies
- hyper/hypocoagulability
- food intolerances
- Irritable bowel syndrome
- Inflammatory bowel disease
- Celiac disease
- pregnancy
We found this trial at
1
site
1211 Medical Center Dr
Nashville, Tennessee 37232
Nashville, Tennessee 37232
(615) 322-5000
Vanderbilt Univ Med Ctr Vanderbilt University Medical Center (VUMC) is a comprehensive healthcare facility dedicated...
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