Pilot Study of Triphala and Rubia Cordifolia on Gut Microbiome and Skin

Herbal supplements such as Rubia cordifolia and Triphala [a mix of Emblica officinalis
(Amalaki), Terminalia bellerica (Bibhitaki), and Terminalia chebula (Haritaki)] are commonly
used for skin based treatments in India. However, the scientific evidence for their specific
effects on the skin are scant. Rubia cordifolia is a root that is used in skin care for
pigmentation and inflammation.1 Triphala is thought to have antioxidant properties and reduce
inflammation in general. Herbs have been shown to modulate the gut microbiome, as previous
studies have suggested that triphala may modify the gut microbiome.2

However, few studies have assessed the effects of these herbs from a skin biophysical
perspective. Here, we aim to understand how these herbs can modulate the skin's barrier
properties and the gut microbiome.

Inclusion Criteria for subjects:

1. Age 18 to 60 years of age

2. Subject must be able to read and comprehend study procedures and consent forms.

Exclusion Criteria for subjects:

1. Subject should be generally healthy and have no smoking history in the past one year.

2. Subjects must have no history of diabetes, metabolic syndrome, known cardiovascular
disease, malignancy, kidney disease, or chronic steroid use.

3. Those who are unable to discontinue topical medications for two weeks.

4. Those who are unable to discontinue systemic antibiotics or oral probiotics for one
month prior starting the study.

5. Those who are unable to discontinue their Triphala and Rubia Cordifolia regimen for
one month prior to starting study.

6. Subjects who are postmenopausal

7. Those who are pregnant or breastfeeding

8. Those that are prisoners or cognitively impaired

9. Those who have a known allergy to Rubia cordifolia, Emblica officinalis, Terminalia
bellerica, Terminalia chebula.