Effect of Nitrate-rich Fruit and Vegetable Supplement on Blood Pressure

Endothelial cells of the peripheral vasculature are key sources of vasoactive factors
regulating healthy blood pressure. One of the primary vasodilatory factors released from
these cells is nitric oxide (NO). Production of NO is stimulated both through
enzymatic-dependent mechanisms via NO synthase, as well as from dietary intake of
nitrate-containing foods or supplements that increase NO bioavailability. This study examined
whether the daily consumption of a nitrate-rich, two-ounce fruit and vegetable energy
supplement (Isagenix International LLC) would increase circulating nitrates and improve
cardiovascular parameters compared to a nitrate-deficient placebo (prune juice). Healthy male
adults aged 18-40y (n=45) were recruited to participate in this longitudinal double-blind,
placebo-controlled, randomized clinical trial. Subjects maintained their typical diet and
physical activity patterns during the study. Anthropometric and cardiovascular (blood
pressure and flow-mediated dilation (FMD)) parameters, along with plasma nitrates and
nitrites were measured at baseline and after one and two weeks of supplementation. Subjects
also completed questionnaires on sleep quality and mood. It is hypothesized that the
nitrate-rich FVS supplement will provide a good source of dietary nitrates and effectively
reduced blood pressure in normotensive, healthy young males.

Inclusion Criteria:

• healthy men aged 18-40y

Exclusion Criteria:

- hypo- or hypertension (blood pressure < 100/65 or >140/90

- cigarette use within past year

- food allergies

- specific medication use (nitroglycerin, beta-blockers, calcium channel blockers),

- unwillingness to drink juice concentrates and follow study restrictions.