Molecular Markers in Thyroid Cancer

The overall objective of this study is to evaluate the prevalence of molecular markers in
patients with benign and malignant thyroid lesions. This study consists of:

Retrospective review of archived surgical pathology specimens at Oregon Health & Science
University (OHSU) from patients with thyroid cancer or benign thyroid disease (nodules or
goiter) who underwent thyroidectomy and/or neck dissection as standard of care. Molecular
markers will be evaluated on archived tissue.

Molecular markers will be correlated with clinical information extracted from OHSU medical
records: histologic subtype of cancer, measures of tumor aggressiveness (capsular and
angiolymphatic invasion, local invasion, lymph node and distant metastases, TNM stage(TNM
Classification of Malignant Tumours)) and clinical outcome (recurrence, distant metastases
and death).

Patients with other malignancies presenting for standard of care services will have
peripheral blood collected for DNA, RNA and buffy coat/white blood cells as a "positive"
control for the DNA/RNA isolation techniques and mutation assays, as other cancers commonly
express some of the same mutations. Normals will have peripheral blood collected for DNA,
RNA and buffy coat/white blood cells as a "negative" control for the DNA/RNA isolation
techniques and mutation assays.

Inclusion Criteria:

- Age 1 - 100

- Benign or malignant thyroid lesion, other malignancy or no thyroid abnormality

- Pathologic specimen available for analysis

- Ability to provide informed consent (for prospective study, Part Two)

- Age greater than age 18 (for normal controls)

Exclusion Criteria:

- Patients without adequate data for analysis

- Histopathologic or cytopathologic diagnosis of for medullary thyroid carcinoma (not
derived from the thyroid follicular epithelium), thyroid lymphoma

- Unwilling to participate or unable to provide informed consent