Haploidentical Allogeneic Peripheral Blood Transplantation: Examining Checkpoint Immune Regulators' Expression

Inclusion Criteria:

- Age: less than 75 years

- The patient must be approved for transplant by the treating transplant physician. This
includes completion of their pre-transplant workup, as directed by standard
Dartmouth-Hitchcock Medical Center (DHMC) Standard Operating Procedure (SOP) (DHMC SOP
- Pre-transplant Evaluation of allogeneic recipient (Appendix).

- The patient must have a disease (listed below) with treatment-responsiveness that the
treating transplant physician believes will benefit from an allogeneic stem cell
transplant. The diseases include:

- Acute leukemia - Acute Myeloid Leukemia, Acute Lymphocytic Leukemia

- Chronic leukemia - Chronic Myeloid Leukemia, Chronic Lymphocytic Leukemia

- Myelodysplasia

- Myeloproliferative disorder

- Myelofibrosis

- Lymphoma - Non-Hodgkin's Lymphoma or Hodgkin's disease

- Plasma cell disorder, including myeloma, Waldenstrom's Macroglobulinemia

- Donor availability- the patient must have an identified RELATED haplo-identical donor

- No Human Immunodeficiency Virus infection or active hepatitis B or C

- Eastern Cooperative Oncology Group performance status: 0-2

- Diffusing capacity of carbon monoxide (DLCO) greater than or equal to 40 % predicted

- Left ventricular ejection fraction greater than or equal to 40%

- Serum bilirubin < 2x upper limit of normal; transaminases < 3x normal at the time of
transplant

- No active or uncontrollable infection

- In female, a negative pregnancy test if experiencing menstrual periods

- No major organ dysfunction precluding transplantation

- No evidence of an active malignancy that would limit the patient's survival to less
than 2 years. (If there is any question, the PI can make a decision).

Exclusion Criteria:

- Psychiatric disorder or a mental deficiency of the patient that is sufficiently severe
to make compliance with the treatment unlikely, and making informed consent
impossible.

- Major anticipated illness or organ failure incompatible with survival from bone marrow
transplant.

- History of refractory systemic infection

DONOR ELIGIBILITY

- Human leukocyte antigen (HLA) haplo-identical matched related.

- The donor must be healthy and must be willing to serve as a donor, based on standard
National Marrow Donor Program (NMDP) guidelines and DHMC SOP - Donor Evaluation
(Appendix)

- The donor must have no significant co-morbidities that would put the donor at marked
increased risk

- There is no age restriction for the donor

- Informed consent must be signed by donor

DONOR EXCLUSION CRITERIA

- The NMDP guidelines for exclusion criteria will be used (Appendix). In addition, the
following donors are NOT eligible:

- Pregnant or lactating donor

- HIV or active Hep B or C in the donor

- Donor unfit to receive G-CSF and undergo apheresis

- A donor with a psychiatric disorder or mental deficiency that makes compliance with
the procedure unlikely and informed consent impossible