Clinical Response to Rhinovirus Challenge

Primary objectives are:

To determine whether RV increases expression of interleukin (IL)-25 transcripts by nasal
epithelial cells in the asthma and AR but not control cohorts at the peak of infection (days
3 and 4).

To determine whether RV increases lower respiratory symptoms in the asthma but not AR and
control cohorts.

To determine whether asthmatics and allergic rhinitics will demonstrate an increased severity
of infection in comparison to control subjects.

Secondary objectives are:

1. To determine whether asthmatic and AR cohorts demonstrate increased IL-25 transcript
expression over the course of RV infection

2. To determine whether asthmatic and AR cohorts demonstrate increased expression of mRNA
transcripts of a type 2 cytokine-inducing profile (IL-33 and thymic stromal
lymphopoietin (TSLP)).

3. To determine whether increased transcript expression of this type 2 cytokine-inducing
profile can be corroborated as increased expression of protein.

4. To determine whether RV infection in the asthma cohort is associated with increases in
biomarkers of inflammation.

5. To determine whether increased severity of RV infection in the asthma and AR cohorts
will be associated with more symptoms.

6. To determine whether increased severity of RV infection in the asthma and AR cohorts is
related to decreased innate immunity.

Inclusion Criteria:

All subjects:

1. Subjects must be able to understand and provide written informed consent.

2. Age 18 to ≤40 years of age, any gender, any racial/ethnic origin

3. Female subjects of childbearing potential must have a negative pregnancy test upon
study entry (day -7) and before each procedure involving pharmacologic interventions
(days 0, 4, and 7).

4. Female (and male) subjects with reproductive potential, must agree to use FDA approved
methods of birth control for the duration of the study such as, but not limited to,
birth control pills, contraceptive foam, diaphragm, IUD, abstinence, or condoms.

5. Participants must be willing to comply with study procedures and requirements.

6. Negative test for serum neutralizing antibody to RV16 at enrollment visit (<1:8)
(Visit 1).

Allergic Rhinitis Subjects:

1. Allergy as determined by ≥1 positive prick skin test (wheal ≥5 mm diameter and 3mm
larger than the diluent control) to Virginia inhalant panel within 5 years, and a
history of symptoms of sneezing, rhinorrhea, pruritus, nasal congestion, and/or
allergic conjunctivitis on natural exposure to relevant allergens.

2. Negative methacholine challenge (less than 20% decline in functional expiratory volume
in 1 second (FEV1) at ≤8mg/ml) within 1 year

3. FEV1 ≥80% predicted, FEV1/FVC ≥80%.

4. No history of wheezing with viral infection in the last 6 years, and no use of rescue
inhalers or long-term controllers for asthma in the last 6 years.

Allergic Asthmatic Subjects:

1. Allergy as determined by ≥1 positive prick skin test (wheal ≥5 mm diameter and 3mm
larger than the diluent control) to Virginia inhalant panel. Subjects are not required
to have allergy symptoms at the time of study. Subjects will report history of
symptoms of sneezing, rhinorrhea, pruritus, nasal congestion, and/or allergic
conjunctivitis on natural exposure to relevant allergens.

2. Asthma determined by physician diagnosis and by a positive methacholine challenge (at
least 20% fall in FEV1 at a methacholine concentration of ≤8 mg/ml) at screening
protocol visit before enrollment (obtained within the past year).

3. Asthma must be controlled as determined by asthma control test (ACT) score ≥20 and
normal lung function (FEV1>70% predicted or FEV1/FVC ratio >75% for subjects with FVC
values between 80 and 87% predicted whose FEV1 values fall below 70%) at Visits 1 and
2.

Exclusion Criteria:

1. Positive test for serum neutralizing antibody to RV16 at enrollment visit (≥1:8)
(Visit 1).

2. Upper airway modified Jackson criteria symptom scores ≥7 at time of inoculation.

3. Chronic heart disease including bradycardia, lung diseases other than asthma, or other
chronic illnesses including epilepsy, peptic ulcer disease, thyroid disease, urinary
tract infection, vagotonia, autoimmune disease, primary or secondary immunodeficiency
or any household contacts who are known to be immune deficient. Any medical conditions
that could be adversely affected by the administration of cholinergic agent.

4. Any use of corticosteroids, leukotriene (LT) modifiers, antihistamines, omalizumab,
theophylline, long-acting anti-muscarinic antagonists (LAMAs), long-acting
beta-agonists (LABAs), nedocromil, cromolyn use on a daily basis within 4 weeks prior
to Visit 1.

5. Current use of ß-blockers or cholinesterase inhibitors (for myasthenia gravis).

6. ß2-agonist use ≥4 days/week in any week or ≥2 nights/month during the month before
Visit 1.

7. Recent (within 1-yr) asthma exacerbation requiring urgent care visit (unless the
treatment involved only the use of a bronchodilator), hospitalization, or oral CCS

8. Intubation or management in the intensive care unit (ICU) for an asthma exacerbation
ever.

9. An upper or lower respiratory tract infection within 2 months prior to enrollment.

10. Previous nasal or sinus surgery within the last 12 months

11. >5 pack-year smoking history or any smoking within the past 6 mos.

12. Hemoglobin <11.5 g/dL for non-African American subjects or hemoglobin < 11.0 g/dL for
African American subjects detected at Visit 1.

13. Laboratory values (other than hemoglobin and absolute neutrophil count (ANC)) measured
at Visit 1 that are considered to be of clinical relevance by the Investigator.

14. Absolute neutrophil count (ANC) <1500 cells/mm3 (or 1.5 K/µL) or absolute lymphocyte
count (ALC) <800 cells/mm3 detected at Visit 1.

15. Use of investigational drugs within 12 weeks of participation

16. Past or current medical problems or findings from physical examination or laboratory
testing that are not listed above, which, in the opinion of the investigator, may pose
additional risks from participation in the study, may interfere with the participant's
ability to comply with study requirements or that may impact the quality or
interpretation of the data obtained from the study.