Glucagon Counterregulation in Type 1 Diabetes

Participation in this study will require three (3) study visits over 12 weeks: one screening
visit lasting 2-3 hours, and two overnight study visits at the university's Clinical Research
Unit (CRU). The two overnight visits will last about 22 hours.

During the CRU admission, all subjects will wear a Continuous Glucose Monitor (CGM) starting
2-3 days prior to the CRU admission and after having a CGM training.

Eligible subjects will be randomized to either insulin- or exercise-induced hypoglycemia
group. Each subject will have two overnight CRU admissions in randomized order: Experimental
(basal pramlintide + 25% reduction of basal insulin) or Control (standard basal insulin
therapy) admissions. During these two admissions, the study team will deliberately induce
hypoglycemia as follows:

Subjects randomized to insulin-induced hypoglycemia admission will receive an insulin
bolus(s) dosed to reach blood sugar of less than 55 mg/dL.

Subjects randomized to exercise-induced hypoglycemia will participate in three 15 minute
exercise bouts (45 minutes total) to lower blood sugar to less than 55 mg/dL.

After hypoglycemia induction, all subjects will receive one and the same standard meal
(lunch) mixed with 1.5 g liquid acetaminophen to measure how quickly acetaminophen is
absorbed to estimate the rate of gastric emptying.

The study team will collect blood samples during the hypoglycemic induction and the gastric
emptying monitoring which will be analysed for levels of various substances used to address
the study goals.

Inclusion Criteria:

- Clinical diagnosis, based on investigator assessment, of type 1 diabetes for at least
5 years and using insulin for at least 5 years

- Use of an insulin pump for at least 6 months with established parameters for basal
rate(s), carbohydrate ratio(s) and insulin sensitivity factor(s) for at least 3
months.

- HbA1c level <10.5% at screening

- Demonstration of proper mental status and cognition for the study

- Investigator has confidence that the subject can successfully operate all study
devices and is capable of adhering to the protocol

Exclusion Criteria:

- Admission for diabetic ketoacidosis in the 6 months prior to enrollment.

- Severe hypoglycemia resulting in seizure or loss of consciousness in the 3 months
prior to enrollment.

- Hematocrit less that the lower limit of normal for the assay.

- Pregnancy, breast-feeding, or intention of becoming pregnant over time of study
procedures

- A known medical condition, which in the opinion of the investigator or designee, would
put the participant or study at risk

- A recent injury to body or limb, muscular disorder, use of any medication, any
carcinogenic disease, or other significant medical disorder if that injury, medication
or disease in the judgment of the investigator will affect the completion of the
protocol

- Current use of some drugs and supplements

- Participation in another pharmaceutical or device trial at the time of enrollment or
during the study

- Basal insulin rates less than 0.01 units per hour

- Diagnosed food allergies that would prohibit the consumption of a standardized meal

- Any reason the study MD considers that the subject is not appropriate for the trial