Assessment of Biomarkers in Patients With Decompensated Heart Failure and Underlying Coronary Artery Disease

This is a cross-sectional, single center exploratory study of plasma biomarker levels in
adults with decompensated heart failure and significant underlying coronary artery disease as
compared with age-matched healthy controls. Subjects will not receive a therapeutic treatment
as part of this study and will undergo a single blood draw.

Inclusion Criteria:

Group 1: Heart Failure with Coronary Artery Disease Subjects

- Symptomatic heart failure (≥ 3 months) with most recently documented LVEF ≤ 40% (Echo
must have been completed in last 12 months prior to enrollment.)

- Significant underlying coronary artery disease as evidenced by:

- previous myocardial infarction

- prior coronary artery bypass graft

- 50% coronary stenosis of one or more arteries and/or

- history of percutaneous coronary intervention with or without stenting

- Age 18 years or older at the first screening visit

Group 2: Healthy Subjects

• Age 18 years or older at the first screening visit and within 20% of mean age of Group 1
Subjects

Exclusion Criteria:

Group 1:

- Documented history of "severe" valvular disease

- Documented history of atrial fibrillation, ventricular fibrillation or ventricular
tachycardia.

- Recent or current use of anticoagulant medications such as fXa inhibitors, direct
thrombin inhibitors, vitamin K antagonists, or heparin. (within 1 month of screening)

Group 2:

- Known significant cardiovascular or hematological condition

- Recent or current use of anticoagulant medications such as fXa inhibitors, direct
thrombin inhibitors, vitamin K antagonists, or heparin. (within 1 month of screening)

- Recent cold or flu symptoms, respiratory infection or surgery (within 2 weeks of
screening)

- History of Asthma

- History of peptic ulcers