GMCI, Nivolumab, and Radiation Therapy in Treating Patients With Newly Diagnosed High-Grade Gliomas

PRIMARY OBJECTIVES:

I. To assess the safety/maximum tolerated dose (MTD) of the combination of aglatimagene
besadenovec (AdV-tk) given intra-cranially at the time of initial tumor resection followed by
valacyclovir (GMCI), nivolumab, and standard of care (radiation therapy [RT]+temozolomide
[TMZ]) in patients with high-grade gliomas (HGG).

PRIMARY OBJECTIVES:

I. To assess the safety/maximum tolerated dose (MTD) of the combination of aglatimagene
besadenovec (AdV-tk) given intra-cranially at the time of initial tumor resection followed by
valacyclovir (GMCI), nivolumab, and standard of care (radiation therapy [RT]+temozolomide
[TMZ]) in patients with high-grade gliomas (HGG).

SECONDARY OBJECTIVES:

I. To evaluate safety and toxicity of this combined treatment regimen. II. To estimate
overall survival. III. To estimate progression free survival. IV. Immune biomarkers,
including serum extracellular vesicles (EVs).

OUTLINE:

Patients undergo tumor resection and receive AdV-tk injection into the wall of the resection
cavity. Patients then receive valacyclovir orally three times per day for 14 days. Beginning
on approximately day 8, patients undergo radiation therapy five days per week for 6 weeks.
Temozolomide will be initiated on approximately day 15 after valacyclovir is completed and
will continue until MGMT methylation status is known. If unmethylated, temozolomide will be
discontinued: these patients will constitute Cohort 1. In Cohort 2 - patients with methylated
MGMT - temozolomide will continue. If methylation status is unable to be determined, those
patients will also continue receiving temozolomide (Cohort 2). Both cohorts will receive
nivolumab intravenously every two weeks for up to 52 weeks in the absence of disease
progression or unacceptable toxicity.

After completion of study treatment, patients are followed up every 2 months for 2 years, and
then every 6 months thereafter.

Inclusion Criteria:

- Patients must have operable brain tumor presumed to be high grade glioma (HGG) based
on clinical and radiologic evaluation, where a gross total surgical resection of the
contrast-enhancing area is intended; pathologic confirmation of HGG must be made at
the time of surgery prior to AdV-tk injection, if not previously determined

- Patients must have a Karnofsky performance status >= 70% (i.e. the patient must be
able to care for himself/herself with occasional help from others)

- Absolute neutrophil count >= 1,500/uL

- Platelets >= 100,000/uL

- Hemoglobin >= 9 g/dL

- Total bilirubin =< 1.5 x institutional upper limit of normal (ULN), (except for
patients with known Gilbert's syndrome who must have normal direct bilirubin)

- Aspartate aminotransferase (AST) serum glutamic-oxaloacetic transaminase
(SGOT)/alanine aminotransaminase (ALT) serum glutamate pyruvate transaminase (SGPT) =<
3.0 x institutional ULN

- Creatinine =< institutional ULN

- Calculated creatinine clearance >= 40 ml/min (use a modified Cockcroft-Gault equation)

- Activated partial thromboplastin time/partial thromboplastin time (APTT/PTT) =< 1.5 x
institutional ULN

- Patients must be able to provide written informed consent

- Patients must have magnetic resonance imaging (MRI) within 14 days of starting
treatment; patients must be able to tolerate MRI

- Women of childbearing potential must agree to have a negative serum pregnancy test
within 24 hours prior to treatment start; women of childbearing potential must agree
to use adequate contraception (hormonal or barrier method of birth control;
abstinence) prior to study entry, for the duration of study treatment, and through at
least 5 months after the last dose of study drug; should a woman become pregnant or
suspect she is pregnant while participating in this study, she should inform her
treating physician immediately; sexually active men of reproductive potential who are
partners of women with reproductive potential must also agree to use adequate
contraception prior to the study, for the duration of study participation, and through
at least 7 months after the last dose of study drug; adequate methods of effective
birth control include sexual abstinence (men, women); vasectomy; or a condom with
spermicide (men) in combination with barrier methods, hormonal birth control or
intrauterine device (IUD) (women)

- Patients must have no concurrent malignancy except curatively treated basal or
squamous cell carcinoma of the skin or carcinoma in situ of the cervix, breast, or
bladder; patients with prior malignancies must be disease-free for >= two years;
patients with low-risk prostate cancer on active surveillance are eligible

- Patients must be able to swallow oral medications

- Patients must not have received prior radiation therapy, chemotherapy, immunotherapy
or therapy with biologic agent (including immunotoxins, immunoconjugates, antisense,
peptide receptor antagonists, interferons, interleukins, tumor infiltrating lymphocyte
[TIL], lymphokine-activated killer [LAK] or gene therapy), or hormonal therapy for
their brain tumor; glucocorticoid therapy is allowed

Exclusion Criteria:

- Patients receiving any other investigational agents are ineligible

- Patients with a history of hypersensitivity or allergic reactions attributed to
compounds of similar chemical or biologic composition to valacyclovir, acyclovir, or
temozolomide are ineligible; the valacyclovir and temozolomide package inserts can be
referenced for more information

- Patients with a history of severe hypersensitivity reaction to any monoclonal antibody
are ineligible

- Patients who require therapy with systemic immunosuppressive drugs except
corticosteroids are ineligible

- Patients with a history of active autoimmune disease requiring treatment in the past 2
years are ineligible

- Patients with uncontrolled intercurrent illness including, but not limited to, ongoing
or active infection, symptomatic congestive heart failure, unstable angina pectoris,
cardiac arrhythmia, active liver disease or active hepatitis, or psychiatric
illness/social situations that would limit compliance with study requirements, are
ineligible

- Pregnant women are excluded from this study; breastfeeding should be discontinued if
the mother is treated with these agents through 1 week after receiving the last dose
of study drugs

- Patients who are known to be human immunodeficiency virus (HIV) positive are
ineligible