Sirolimus, Tacrolimus and Short Course Methotrexate for Prevention of Acute GVHD in Recipients of Mismatched Unrelated Donor Allogeneic Stem Cell Transplantation

Inclusion Criteria:

- Patients must have an identified 8/10 or 9/10 matched unrelated donor identified
following a formal search with confirmatory typing through the national marrow donor
program as the best available donor. No matched sibling or fully matched unrelated
donor has been identified. HLA typing of donor and recipient will be performed by
high resolution molecular typing at HLA A, B, C and DRB1/DQ loci. Patients whose best
available donor is matched at 8/10 loci must have at least one of the mismatches at
the DQ locus. (no more than one mismatch at HLA A,B,C,DR allowed).

- Candidates for this trial will meet the following criteria:

1. Adequate organ function for conditioning type:

For patients receiving ablative conditioning

- Left Ventricular ejection fraction >45%

- DLCO >50%

- Creatinine <1.5

- Hepatic enzymes <3x upper limit of normal.

- KPS >70%

For patients receiving non-ablative conditioning:

- KPS >70%

2. Patients with the following diseases will be considered eligible:

- AML in first remission with high risk features (poor risk cytogenetic
abnormalities9, persistent elevated blast count on day +15 or recovery
marrow after induction therapy).

- AML beyond first remission

- ALL in first remission with high risk features (ph+, t4:11)

- ALL beyond first remission

- High risk Myelodysplasia (RAEB-II, RAEB-I with poor-risk cytogenetics)

- Recurrent Aggressive Non-Hodgkins or Hodgkins lymphoma (indolent
histologies excluded) who have failed autologous transplant or have had
inadequate response to salvage therapy.

- CML with transformation

- CLL with transformation or Fludarabine failure.

- Severe aplastic anemia with recurrence or failure after immunosuppressive
therapy.

Exclusion Criteria:

- Prior allogeneic transplantation

- Active CNS leukemia.

- Female patients who are pregnant or breast feeding

- Karnofsky performance status <70%.

- Active viral, bacterial or fungal infection.

- Patients seropositive for HIV -1,2; HTLV -1,2 (due to the additional immunodeficiency
induced by transplantation and immunosuppressive therapy) Requirement for antifungal
prophylaxis with Voriconazole for the first 30 days is prohibited.

- Patients not providing informed consent.