Prospective Phenotyping of Autonomous Aldosterone Secretion

Primary aldosteronism is a disorder wherein aldosterone is secreted by the adrenal gland(s)
independent of its physiologic regulators and cannot be appropriately suppressed with
sodium/volume loading. Primary aldosteronism is a common cause of hypertension and has a
relatively high prevalence. This is important since the excessive mineralocorticoid receptor
activation in primary aldosteronism contributes to adverse cardiovascular and renal outcomes
and death. For these reasons, it is critical that autonomous aldosteronism be detected early
in its course since appropriate treatment interventions may prevent cardiovascular disease.

In addition to severe and overt primary aldosteronism in hypertension, human studies have
shown that milder forms of primary aldosteronism can exist even among normotensive
individuals. Detailed physiologic studies have shown that normotensive individuals with a
phenotype of autonomous aldosterone secretion have greater cardiometabolic risk factors,
impaired renal-vascular function, and a higher risk for developing incident hypertension.
Further, older age is associated with greater autonomous aldosterone secretion, suggesting
that autonomous aldosterone secretion may progress over time. A better understanding of the
prevalence and progression of this type of "subclinical" autonomous aldosterone secretion may
inform our understanding of the pathogenesis of hypertension and cardiometabolic diseases.

This protocol is designed to be a prospective longitudinal study that will carefully
characterize the degree of autonomous aldosterone secretion among high-risk normotensive
individuals and follow them longitudinally with repeated phenotyping study visits to assess
the progression and severity of autonomous aldosterone secretion over time and its relevance
to cardiovascular health. Phenotyping visits will include measurements of the
renin-angiotensin-aldosterone system under controlled posture and variable sodium intakes,
adrenal and vascular responses to angiotensin II, renal blood flow measurements, and repeated
assessments of blood pressure.

This prospective cohort study will provide insights into normal and abnormal aldosterone
physiology over time and how it may contribute to time- or age-dependent hypertension and
cardiometabolic risk.

Inclusion Criteria:

1. Age 35-70 years

2. Systolic blood pressure of 120-135 mmHg and/or diastolic blood pressure of 75-85 mmHg

3. At least one, or more, of the following:

- BMI ≥ 25 kg/m2

- Family history of hypertension prior to the age of 60 years in a parent or
sibling

- Diabetes with a hemoglobin A1c < 9%

4. If systolic blood pressure 115-135 mmHg and/or diastolic blood pressure 70-85 mmHg,
must have two or more of the following:

- BMI ≥ 25 kg/m2

- Family history of hypertension prior to the age of 60 years in a parent or
sibling

- Diabetes with a hemoglobin A1c < 9%

Exclusion Criteria:

- Known history of hypertension or use of antihypertensive medications

- Known history of stroke, coronary artery disease, myocardial infarction, heart
failure, cerebral or aortic aneurysm, or preeclampsia.

- Active cancer that is being treated with chemotherapeutic agents

- Pregnancy

- Breast feeding

- Daily use of prescribed opioid medications

- Illicit drug use (cocaine, heroin, methamphetamine)

- Daily non-steroidal anti-inflammatory medication use

- Daily use of glucocorticoids

- Electrocardiogram that shows evidence of prior myocardial infarction, atrial
arrhythmia, left or right bundle branch blocks.

- Estimated glomerular filtration rate < 60 mL/min/1.73m2

- Active and untreated hyper- or hypo-thyroidism

- Abnormal screening laboratories (comprehensive metabolic panel, complete blood count,
thyrotropin)

- BMI ≥ 45 kg/m2