Ethanol Induces Skeletal Muscle Autophagy

Hypothesis: Ethanol mediated modification of skeletal muscle proteins to form MAA adducts
that in turn induce skeletal muscle autophagy.

Patients with alcoholic steatosis, hepatitis and cirrhosis (n=10 each) will be recruited from
the liver transplant nutrition clinic or the hepatology inpatient service and their body
composition quantified using anthropometry, bioelectrical impedance analysis, CT image
analysis and DEXA if available.

Control Subjects. Controls will be recruited by advertisement. All control subjects will have
a normal clinical history, physical examination, and screening chemistries. They will not
have any significant medical conditions requiring the use of medications. Their nutritional
status within 20% of normal as defined by ideal body weight.

Inclusion Criteria:

Alcoholic liver disease:

- clinical, biochemical, imaging criteria and liver biopsy where available

- Age 18 - 65 years old

Controls:

- Serum liver transaminases (i.e. ALT and AST) 40 IU/L

- Normal liver ultrasound

- Age 18 - 65 years old

Exclusion Criteria:

For both groups - alcoholic liver disease and controls:

- Poorly controlled diabetes mellitus (HbA1C>9.5 g/dl)

- Untreated Hyper- / hypo- thyroidism

- Patients on dialysis, renal disease with serum creatinine 1.5 mg/dL

- Active intravenous drug use

- History of bowel surgery or gastric bypass surgery

- Medications known to alter muscle protein metabolism (i.e. corticosteroids, tamoxifen,
high-dose estrogen, testosterone or anabolic steroids)

- Metastatic disease, Advanced cardiac or pulmonary disease

- Pregnancy

- Coagulopathy- INR >1.4 and platelet count <80,000/ml.