The Effects of Dietary Nitric Oxide and Anthocyanins on Anaerobic Exercise Performance

A randomized controlled double-blind crossover study with a baseline and two interventions.
On day 1 (baseline), all subjects will have a lactate fingerstick measurement before and
after the Wingate protocol. A random subsample (1/3 of subjects) will have 10 milliliters (2
teaspoon) of blood drawn for determination of serum allantoin. Subjects will then be equipped
with a pulse oximeter and a heart rate monitor after which they will perform the Wingate
Anaerobic test protocol. The protocol includes: 4 minutes of warm up, 3 seconds of warm up
sprint with weights, 1-minute relaxed cycling, 2-minute rest, then 10 seconds cycling without
weights. This will be followed by 30 seconds sprint with weights and end with 2 minutes of
relaxed cycling. A post-activity blood draw will be performed for another 10 milliliters (2
teaspoon) of blood from the same one-third of subjects previously sampled. After baseline
data collection, subjects will undergo a 1-week washout (avoiding high antioxidant foods)
after which they will be randomized to treatment groups. On day 9, subjects will begin
supplementation with nitrate from beetroot powder with additional anthocyanins, or nitrate
from beetroot powder with a placebo for 7 days. On day 15 the subject will again repeat the
same procedures as day 1, followed by another week of washout. They will supplement again on
day 23 and repeat the same procedure as the first intervention on day 29.

Inclusion Criteria:

- Healthy Loma Linda University students aged between 21 and 35 years old

- Willing to abstain from caffeine, mouthwash, chewing gums, and high antioxidant foods
during the week of supplementation.

Exclusion Criteria:

- Diabetic

- having salivary gland disorder, cardiovascular disease or orthopedic injuries

- pregnant or breastfeeding

- taking NSAIDS

- have an allergy to anthocyanins and/or beetroot

- women who are on their menses