A Comparison of High-intensity Interval Training Protocols on Health and Fitness

The PI will verify age with a drivers license or other form of identification to ensure that
the study participants are at least 18 years of age to participate. After obtaining consent,
the PI will then administer a pre-exercise screening tool, the Physical Activity Readiness
Questionnaire for Everyone, to the participant for them to complete in order to aid in
determining study eligibility. If the participant is deemed eligible, 3 other questionnaires
will be emailed to participants for completion: Physical Activity Enjoyment Scale, Exercise
Self-efficacy Scale, and the International Physical Activity Questionnaire (IPAQ) short form.
Given that the participants will be instructed to not change any physical activity habits
outside of the study, the IPAQ will be used to control for this pre- and post-intervention
and weekly throughout the duration of the intervention. All forms must be completed and
submitted to the PI prior to further data collection. Data collection will consist of a (1)
blood collection visit, (2) exercise testing and abdominal/visceral fat measurement session,
(3) three exercise/movement familiarization sessions (only conducted pre-intervention), (4)
three strength, muscular endurance, and muscular power testing sessions before and after the
intervention, and 12 weeks of training three times per week as described below. Excluding the
consent obtaining meeting, participants will attend 8 sessions prior to beginning the
intervention as detailed below. During the intervention, participants will be wearing heart
rate monitors in order to monitor their training intensity. The PI will lead all data
collection procedures with the assistance of School of Health Science students and Dr. Myung
Choi except for the blood collection and analysis which will be supervised by Dr. Kristen
Landis-Piwowar. All measures will be assessed pre- and post-intervention. Blind copy emails
will be sent to remind the participants about upcoming data collection sessions and to
schedule a data collection appointment.

Day 1: Prevention Research Center (blood collection visit)

Following 12-hr fast, assessments include:

- weight, height, waist circumference

- resting heart rate

- blood pressure

- venipuncture (blood sugar and fat)

- blood analysis

Day 2: Prevention Research Center (exercise testing and visceral fat visit)

Assessments include:

- dual-x-ray absorptiometry (DXA) to measure visceral adipose tissue

- bicycle VO2max test with standard warm-up and clinical monitoring to assess aerobic
endurance

- Physical Activity Enjoyment Scale and Exercise Self-efficacy Scale (post-intervention)

Days 3-5: Oakland University (OU) Recreation Center (familiarization visits)

Familiarization sessions include:

- teaching movements used in subsequent testing and training

- assessment of musculoskeletal limitations

- intervention leader and assistants will attend sessions as part of training

Days 6-8: OU Recreation Center (performance testing visits)

Assessments include:

- Day 6: one repetition maximum (1 RM) testing for back squat (muscular strength); 70% 1RM
back squat for max effort repetitions unbroken (muscular endurance)

- Day 7: 1 RM overhead press (muscular strength)

- Day 8: 1 RM deadlift (muscular strength) and max broad jump (muscular power)

- standardized warm-up prior to cool-down following each session

- at least 48 hours of rest between each session

Pre- and post-intervention health measurements: Prevention Research Center Anthropometrics
and vital sign measures such as height (using a stadiometer), weight (using an eye-level beam
scale), heart rate and blood pressure (sitting with a sphygmomanometer) will be obtained.

Pre- and post-intervention endurance measurements: Prevention Research Center Aerobic fitness
will be directly assessed by a VO2max bicycle test. Each participant will be allowed to
warm-up on the bicycle for 5 minutes at a low intensity. The VO2max bicycle test will include
3 minute stages with increasing intensity each stage of 30 - 60 watts. The required cadence
will be maintained at 60 revolutions per minute (RPM) for each stage. The test will be
terminated at volitional fatigue defined as the participant no longer able to maintain 60
RPMs for 20 seconds after encouragement from the testers. For most people, the test will take
approximately 10 minutes.

Pre- and post-intervention strength measurements: OU Recreation Center Prior to strength
testing, three familiarization sessions will be conducted whereby participants will be taught
and given a chance to practice the movements to be used for testing and training. During
these sessions, participants will be assessed for any musculoskeletal limitations such as a
lack of hip and ankle mobility that may require movement/exercise modifications to ensure
safe technique. Following the familiarization sessions, each participant will complete three
further days of testing, with at least 48 hours between testing days. These testing sessions
will occur at OU Recreation Center. Each of these test sessions will be preceded by a
standardized warmup and instruction regarding the upcoming session. On separate days, muscle
strength will be assessed using 1-repetition maximum (1RM) testing for back squat, press, and
deadlift using standardized protocols. The back squat will be performed to below parallel
(hip crease below the apex of the patella), the press will be initiated with the bar on the
clavicles, and the deadlift will performed in the conventional stance. All testing days will
be repeated following training.

Pre- and post-intervention muscle endurance and muscle power: OU Recreation Center Muscle
endurance will be assessed by repetitive back squats to voluntary exhaustion. The load will
be set at 70% of the pre-training

1RM back squat for both pre- and post-training tests. This test will be conducted immediately
after the 1 RM testing for back squat. Movement standards will be the same as those for the
1RM test, and the total number of successful repetitions achieved without a rest will be
recorded. Muscle power will be assessed using a static broad jump. The best horizontal
distance achieved out of 3 trials will be recorded. This test will be conducted immediately
after the 1 RM testing for the deadlift.

Pre- and post-intervention biochemistry measurements: Prevention Research Center Following a
12-hour fast, participants will complete venipuncture collection procedures. Two invasive
needle sticks will be performed during this investigation (one needle stick pre-intervention
and another needle stick post-intervention). Four milliliters (mL) of blood will be collected
both pre- and post-intervention for measurement of: total cholesterol, high-density
lipoprotein cholesterol, triglycerides, alanine aminotransferase, aspartate aminotransferase,
glucose, and low-density lipoprotein cholesterol. This total amount of blood (8mL) collected
during this trial will equate to 1.6 teaspoons of blood (5mL = 1 teaspoon). The risks of
blood draw may include: infection, delayed healing, bruising and/or inflammation at the site
of vein puncture, physical discomfort, mental discomfort, fainting and feeling faint and
injury to a nerve or vessel. These risks will be minimized by the use of a trained
professional (Dr. Kristen Landis-Piwowar) experienced with performing the blood draws,
sterile technique and single use, disposable, materials who will be supervising the blood
draw and analysis. Study participants may refuse to participate at any time and withdraw from
the study without penalty.

Pre- and post-intervention visceral fat measurement using dual x-ray absorptiometry (DXA)
scan: Prevention Research Center For this measurement of visceral fat, each participant will
be asked to lie flat on a special table below the DXA scan. A trained technician (Elise
Brown, Ryan Tyler, Myung Choi, or Samantha Orr) will position each participant's body under
the scan and then perform the scan, which will only take < 5 minutes.

Pre- and post-intervention psychosocial questionnaires The purpose of including these
psychosocial assessments in the proposed study is to assess changes in physical activity
enjoyment and exercise self-efficacy. High-intensity interval training has been shown to be
more enjoyable than continuous training, and this may lead to enhanced self-efficacy. These
questionnaires will be emailed to participants for completion: Physical Activity Enjoyment
Scale and Exercise Self-efficacy Scale.

Nine months following the intervention, participants will complete all assessments again.

The intervention will be led by students (one leader and at least 2 assistants) who will be
trained by the PI, and exercise protocols are as follows:

MM-HIIT Group: This group will train using multimodal exercises for 5 sets of 60 seconds of
all out work, followed by 3 minutes of rest (a total of 20 minutes each session). Each
session will have the same exercises within each set, but every session will be different
than the others for movements utilized. There will be three movements used in each set. The
first movement will be a strength movement (such as overhead press, squat, deadlift) for 4-6
repetitions. The second movement follows the first immediately and is a faster body weight or
light implement power movement such as lunges, body weight squats, or push ups for 6-8
repetitions. The third movement follows the second immediately and is a very fast,
sprint-like movement such as box jumps, ball slams, or jump rope for the remainder of the 60
seconds. The intent of each set is to be completed with as much effort as possible across the
full 60 seconds. The intervention leader will record weight used for each exercise and will
instruct participants to increase intensity (up to 110% of previous intensity used) at
subsequent session once maximum prescribed repetitions have been.

R-HIIT Group: This group will train using a rowing ergometer for 5 sets of 60 seconds of all
out work, followed by 3 minutes of rest (a total of 20 minutes each session). Each training
session will be the same across the 12 weeks. The intent of each set is to be completed with
as much effort as possible across the full 60 seconds.

Control Group: This group will be instructed to continue with any activity they were involved
in prior to the study and to not begin any new exercise programs during the course of the
study. To incentive participation in this group, the control group will be offered the
MM-HIIT intervention the following semester at no cost.

Inclusion Criteria:

- Potential participants will be non-obese (waist circumference ≤ 88 cm) recreationally
active females aged 18 - 40 years who do not engage in a regular training program.
Recreationally active is defined as periodically participating in physical activity or
exercise between 1 - 3 hours a week for at least a month, but not involved in a
systematic endurance or weight training activity.

Exclusion Criteria:

- Potential participants will be excluded if they have a history of exercise-limiting
cardiovascular, respiratory, metabolic, or musculoskeletal illness/injury or if they
are currently taking medication that would alter the physiological responses to
exercise. They will also be excluded if they are pregnant or have a history of
fainting while giving blood.