ONES Grant: CXCL10/CXCR3 Regulation of Ozone-Induced Epithelial Permeability
| Status: | Recruiting |
|---|---|
| Healthy: | No |
| Age Range: | 18 - 45 |
| Updated: | 10/18/2018 |
| Start Date: | September 20, 2018 |
| End Date: | August 2020 |
| Contact: | Catherine Foss |
| Email: | catherine.foss@dm.duke.edu |
| Phone: | 1-919-479-0861 |
CXCL10/CXCR3 Regulation of Ozone-Induced Epithelial Permeability
The purpose of this research study to understand how environmental and genetic factors may be
involved in lung function. Study participants will undergo a 1-day screening that includes a
blood draw and breathing testing, return for a two-day series of testing to include blood
draw, and brief breathing test before and after an inhaled challenge with either filtered air
(FA) or ozone (O3). Participants return the next day for a brief breathing test, a blood draw
and a procedure called bronchoscopy to evaluate the lung after the challenge. Participants
then return 18 - 20 days later to repeat the two-day series of testing to be challenged with
the exposure not received on the first series, (FA or O3). Each visit will take about 3 - 3.5
hours. Follow-up phone calls from the study team will occur at 24 hours after each 2-day test
series. Total study duration is about one to one-and a half months.
involved in lung function. Study participants will undergo a 1-day screening that includes a
blood draw and breathing testing, return for a two-day series of testing to include blood
draw, and brief breathing test before and after an inhaled challenge with either filtered air
(FA) or ozone (O3). Participants return the next day for a brief breathing test, a blood draw
and a procedure called bronchoscopy to evaluate the lung after the challenge. Participants
then return 18 - 20 days later to repeat the two-day series of testing to be challenged with
the exposure not received on the first series, (FA or O3). Each visit will take about 3 - 3.5
hours. Follow-up phone calls from the study team will occur at 24 hours after each 2-day test
series. Total study duration is about one to one-and a half months.
Inclusion Criteria:
- Prior enrollment in the NIEHS Environmental Polymorphism Registry located in the
greater Raleigh-Durham area
- Wild type or homozygous (female)/hemizygous (male - since sex-linked) expression of
the CXCR3 polymorphism rs2280964
Exclusion Criteria:
- Current smokers of tobacco products including e-cigarettes or those with previous
smoking history within the prior 5 years
- BMI > 35 kg/m2
- Pregnant women and women who are presently lactating.
- Subjects that have received antibiotic administration or an upper respiratory
infection within the previous 4 weeks
- College and graduate students or employees who are under direct supervision by any of
the investigators in this protocol
- Alcohol or illicit substance abuse
- Chronic cardio/pulmonary respiratory disorders or other medical conditions as
determined by the investigator
- Increased airway hyperresponsiveness at baseline as measured by a positive
methacholine challenge response (methacholine PC20 FEV1 < 8 mg/ml)
- Subjects will be requested to refrain from antihistamines, nonsteroidal
anti-inflammatory agents, antioxidants (e.g. beta-carotene, selenium, and lutein) and
supplemental vitamins (e.g. C and E), for 1 week prior to, and during testing.
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