United States Pre-Market Tobacco Application Pharmacokinetics

E-cigarettes may be an acceptable alternative to traditional cigarette smoking. In utilizing
vaporization rather than combustion, the generation and inhaltion of smoke and carbon
monoxide (CO) may be reduced or avoided. JUUL has developed several nicotine based liquid
blends for use in e-cigarettes. This study will assess nicotine uptake during a 10-puff
controlled and a 5-minute ad libitum use of four JUUL 5% electronic nicotine delivery systems
(ENDS) relative to usual brand (UB) combustible cigarettes, a comparator electronic cigarette
(e-cigarette), and nicotine gum.

Inclusion Criteria:

- Healthy, adult, male or female smoker, 21 to 65 years of age, inclusive

- Has been a smoker for at least 12 months prior to Screening. Brief periods of
non-smoking (e.g., up to ~7 consecutive days due to illness, trying to quit,
participation in a study where smoking was prohibited) during that time will be
permitted at the discretion of the PI.

- Currently smokes an average of 10 or more king size or 100s manufactured combustible
CPD, as reported at Screening.

- Has a positive urine cotinine (≥ 500 ng/mL) at Screening

- Has an exhaled CO > 12 ppm at Screening.

- Is willing to comply with the requirements of the study, including a willingness to
use the study products during the study.

Exclusion Criteria:

- Has a history or presence of clinically significant gastrointestinal, renal, hepatic,
neurologic, hematologic, endocrine, oncologic, urologic, pulmonary, immunologic,
psychiatric, or cardiovascular disease, or any other condition that, in the opinion of
the PI, would jeopardize the safety of the subject or impact the validity of the study
results.

- Has a clinically significant abnormal finding on the physical examination, medical
history, vital signs, ECG, or clinical laboratory results, in the opinion of the PI.

- Has a positive test for human immunodeficiency virus (HIV), hepatitis B surface
antigen (HBsAg), or hepatitis C virus (HCV) at Screening.

- Has had an acute illness (e.g., upper respiratory infection, viral infection)
requiring treatment within 14 days prior to Check-in.

- Has a fever (> 100.5°F) at Screening or Check-in.

- Has a body mass index (BMI) > 40.0 kg/m2 or < 18.0 kg/m2 at Screening.

- Has a history of drug or alcohol abuse within 24 months of Check-in, as determined by
the PI.

- Has diabetes mellitus, asthma, or chronic obstructive pulmonary disease.

- Has a systolic blood pressure < 90 mmHg or > 150 mmHg, diastolic blood pressure < 40
mmHg or > 95 mmHg, or heart rate < 40 bpm or > 99 bpm at Screening.

- Has experienced an allergic reaction following previous e-cigarette use or with
exposure to any primary components of the e-liquids (benzoic acid, propylene glycol
and glycerol).

- Has an estimated creatinine clearance < 80 mL/minute (using the Cockcroft-Gault
equation) at Screening.

- Has a positive urine screen for alcohol or drugs of abuse at Screening or Check-in.

- Has used medications known to interact with cytochrome P450 (CYP) 2A6 (including, but
not limited to, amiodarone, amlodipine, amobarbital, buprenorphine, clofibrate,
clotrimazole, desipramine, disulfiram, entacapone, fenofibrate, isoniazid,
ketoconazole, letrozole, methimazole, methoxsalen, metyrapone, miconazole, modafinil,
orphenadrine, pentobarbital, phenobarbital, pilocarpine, primidone, propoxyphene,
quinidine, rifampicin, rifampin, secobarbital, selegiline, sulconazole, tioconazole,
tranylcypromine) within 14 days or 5 half-lives of the drug, whichever is longer,
prior to Check-in.

- Has used nicotine-containing products other than manufactured cigarettes (e.g., ENDS,
roll-your-own cigarettes, bidis, snuff, nicotine inhaler, pipe, cigar, chewing
tobacco, nicotine patch, nicotine spray, nicotine lozenge, or nicotine gum) within 14
days prior to Check-in.

- Has a prior history of JUUL product use prior to Screening

- Has used any prescription smoking cessation treatments, including, but not limited to,
varenicline (Chantix®) or buproprion (Zyban®) within 3 months prior to Check-in.

- Is a self-reported puffer (i.e., adult smokers who draw smoke from the cigarette into
the mouth and throat but do not inhale).

- Is planning to quit smoking during the study or postponing a quit attempt in order to
participate in the study.

- Has donated plasma within 7 days prior to Check-in.

- Has donated blood or blood products (with the exception of plasma as noted above), had
significant blood loss, or received whole blood or a blood product transfusion within
56 days prior to Check-in.

- Has participated in a previous clinical study for an investigational drug, device,
biologic, or tobacco product within 30 days prior to Check-in.

- Is or has a first-degree relative (i.e., parent, sibling, child) who is a current
employee of the study site.

- Is or has a first-degree relative (i.e., parent, sibling, child) who is a current
employee, shareholder, or is member of the board of directors of JUUL Labs Inc.

- Has previously been diagnosed with any form of cancer, except for basal cell or
squamous epithelial carcinomas of the skin that have been resected at least 1 year
prior to Screening.