BFR and Muscle Mitochondrial Oxidative Capacity
| Status: | Recruiting |
|---|---|
| Conditions: | Orthopedic |
| Therapuetic Areas: | Orthopedics / Podiatry |
| Healthy: | No |
| Age Range: | 18 - 40 |
| Updated: | 2/28/2019 |
| Start Date: | January 28, 2019 |
| End Date: | August 31, 2020 |
| Contact: | Brian Irving, PhD |
| Email: | brianairving@lsu.edu |
| Phone: | 225-578-7179 |
Impact of Low-Intensity Resistance Exercise With and Without Blood Flow Redistricted (BFR) on Muscle Mitochondrial Oxidative Capacity
Blood flow restricted (BFR) exercise has been shown to improve skeletal muscle adaptations to
resistance exercise. BFR uses blood pressure cuffs (i.e., tourniquets) to reduce skeletal
muscle blood flow during resistance exercise. One benefit of BFR is that skeletal muscle
adaptations to resistance exercise training including muscle hypertrophy and increases in
strength can be achieved at lower-loads (e.g., 25-30% 1RM), that are often comparable to more
traditional resistance training loads (70-85% 1RM). However, the impact that low-load BFR
resistance exercise has on muscle quality and bioenergetics is unknown. The present study
will examine the impact of 6 weeks of low-load, single-leg resistance exercise training with
or without personalized BFR on measures of muscle mass, strength, quality, and mitochondrial
bioenergetics. The investigators will recruit and study up to 30, previously sedentary,
healthy, college-aged adults (18-40 years). The investigators will measure muscle mass using
Dual Energy X-Ray Absorptiometry and muscle strength and endurance using isokinetic testing.
The investigators will normalize knee extensor strength to lower limb lean mass to quantify
muscle quality. The investigators will also use near infrared spectroscopy (NIRS) to measure
mitochondrial oxidative capacity in the vastus lateralis. Finally, the investigators will
measure markers of systemic inflammation and markers of muscle damage using commercially
available ELISA assays.
resistance exercise. BFR uses blood pressure cuffs (i.e., tourniquets) to reduce skeletal
muscle blood flow during resistance exercise. One benefit of BFR is that skeletal muscle
adaptations to resistance exercise training including muscle hypertrophy and increases in
strength can be achieved at lower-loads (e.g., 25-30% 1RM), that are often comparable to more
traditional resistance training loads (70-85% 1RM). However, the impact that low-load BFR
resistance exercise has on muscle quality and bioenergetics is unknown. The present study
will examine the impact of 6 weeks of low-load, single-leg resistance exercise training with
or without personalized BFR on measures of muscle mass, strength, quality, and mitochondrial
bioenergetics. The investigators will recruit and study up to 30, previously sedentary,
healthy, college-aged adults (18-40 years). The investigators will measure muscle mass using
Dual Energy X-Ray Absorptiometry and muscle strength and endurance using isokinetic testing.
The investigators will normalize knee extensor strength to lower limb lean mass to quantify
muscle quality. The investigators will also use near infrared spectroscopy (NIRS) to measure
mitochondrial oxidative capacity in the vastus lateralis. Finally, the investigators will
measure markers of systemic inflammation and markers of muscle damage using commercially
available ELISA assays.
Inclusion Criteria:
1. Capable and willing to give written informed consent
2. Capable of understanding inclusion and exclusion criteria
3. 18-40 years of age inclusive
4. Body Mass Index (BMI) between 18.5-25 kg/m2 inclusive
5. Currently untrained
6. No medical condition that would limit their participation in supervised exercise
training based on the Physical Activity Readiness Questionnaire for Everyone (PARQ+)
7. No current prescription medications, except birth control
8. Willing to allow researchers to use data, biological specimens (e.g., blood) and
images (e.g., Dual Energy X-Ray Absorptiometry) for research purposes after study
participation is completed
Exclusion Criteria:
1. Evidence or self-report being pregnant, lactating, or anticipating becoming pregnant
in the next year
2. Participation in resistance or aerobic exercise training > 2 days per week within the
3 months prior to screening
3. Self-report of history of type 1 or 2 diabetes mellitus
4. Self-report history of cardiovascular, peripheral vascular, cerebral vascular,
pulmonary, or renal disease
5. Self-report or evidence of uncontrolled hypertension
6. Self-report history of blood clotting disorders
7. Self-report history of deep vein thrombosis or pulmonary embolism
8. Self-report history of sickle cell trait
9. Self-report history of varicose veins
10. Self-report history of a myopathy leading to muscle loss, weakness, severe cramps or
myalgia
11. Self-report history of orthopedic limitations that would preclude them from
participation in a dynamic exercise program
12. Self-report history of musculoskeletal disorders (e.g., severe osteoarthritis,
rheumatoid arthritis, avascular necrosis or osteonecrosis)
13. Self-report history of neurological disorders (e.g., peripheral neuropathy,
amyotrophic lateral sclerosis, multiple sclerosis, fibromyalgia, Parkinson's disease)
14. Weight loss of > 10% in the last 3 months prior to screening
15. Active smoking
16. Current consumption of > 14 alcoholic drinks per week based on self-report
17. Absolute Contraindication to Exercise as Defined by the American College of Sports
Medicine,1 including:
1. Resting diastolic blood pressure > 100 mm Hg
2. Resting systolic blood pressure > 180 mm Hg
3. Resting heart rate > 100 beats per min
18. Self-report acute viral or bacterial upper or lower respiratory infection at screening
19. Any other condition that in the judgment of the Principal Investigator and/or the
Medical Director of this protocol may interfere with study participation and adherence
to the protocol
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