Reactions to E-cigs and Alcohol

Participants will be recruited via study fliers and notices in the community and via
word-of-mouth. After viewing study advertisements, participants will call the lab and
complete an over the phone screening questionnaire (approximately 10 minutes) with a trained
research assistant (trained by PI, completed CITI training). If qualified participants will
schedule two sessions (each approximately 4 hours for each session) to come to the lab and
complete the study. Participants will receive an appointment reminder by e-mail approximately
48 hours prior to their scheduled study time. Two sessions will be scheduled (in
counterbalanced order): One in which the participant will be exposed to e-cigarette cue and
be allowed to use their e-cigarette during the alcohol taste test (e-cig cue session) and one
in which they will not be exposed to e-cig cues or be allowed to use their e-cig during the
alcohol taste test (no e-cig cue session). Participants will be asked to not schedule any
sessions on days or the day before they have obligations (e.g. tests, work) in order to help
ensure their participation in the study does not affect outside obligations. Participants
will be paid $100 for study completion

After qualifying for the study in the phone screen, participants will complete two separate
counterbalanced sessions: the e-cig cue session and the no e-cig cue session.

1. Participants will report to the lab where they will undergo informed consent process.
Then, the researcher will ask the person to rinse their mouth with water and then will
administer a breath alcohol test using a Breathalyzer, by asking the participant to
breathe into the breathalyzer for approximately 10 seconds. The breathalyzer produces an
immediate reading of BrAC. If > 0.00, a second rinse and reading will be conducted to
avoid false positive readings. If positive on the second reading, participants will be
excluded from the study, but the participant will not be able to leave the lab until
their BrAC falls below 0.02. Participants will then be escorted to a private bathroom
and will be asked to give a urine sample in a small cup. The research assistant will
then use drug and pregnancy (women only) "dip stick" tests to determine pregnancy or
drug use. Results are given within 2 minutes. If positive on either, a second test will
be conducted on the same urine sample to avoid false positive readings. If positive
again, Dr. Melissa Cyders, HSPP, who will be on call for the study, will be contacted.
Dr. Cyders will use her expertise in clinical psychology and she and the research
assistant will inform the participant of the positive test and provide the individual
with any resources they would like (e.g. obstetrician referrals, physical examination
appointment setting, drug use referrals). In case of two positive screens for pregnancy
or for any drug other than marijuana, participants will be dismissed from the study.
Informed consent and initial screening process takes approximately 15 minutes.

2. Participants will fill out the following surveys on a computer about their substance use
and other psychological factors: The UPPS-P Impulsive Behavior Scale, the Alcohol Use
Disorder Identification Test, the Nicotine and Other Substance Interaction Expectancies
Questionnaire E-cig Revised, The Alcohol Expectancy Questionnaire, the Smoking
Consequences Questionnaire, the Timeline Follow Back, the Fagerstrom test for Nicotine
Dependence, Demographics, current e-cig craving, current alcohol craving, current mood
assessment and Smoking Assessments. (approx 25 45 min)

3. Participants will complete either the e-cig cue session below or the no-e-cig cue
session below (one in session 1 and the other in session 2):

1. In the e-cig cue session, participants will be asked to bring their own e-cig and
an e-cig refill liquid or cartridge. Researchers are telling participants that the
study is examining the effect of nicotine use on cognitive acuity. Participants
will then be asked to take 10 puffs from their e-cig over a five-minute period,
consistent with findings on average puffs per use.Then, participants will report
e-cig and alcohol craving and mood. Following this rating, all participants will
then complete the visual dot probe task with eye tracking, using an Eye-Trac D6
Desktop mounted camera. Participants will face a computer, approximately 24 inches
from the computer screen, and their eye movements will be calibrated to ensure
accuracy of eye movement tracking. For the dot probe task, participants will first
see a pair of alcohol-related pictures and matched control pictures, (approximately
7 x 5 inches as used in previous work) which will be presented for 1000ms. After
picture offset, a visual probe appears where one of the pictures had previously
been presented and participants will be instructed to identify the location of the
probe as quickly as possible by pressing either the left or right mouse button.
Following completion of the dot-probe tasks, participants will self-report alcohol
and e-cig craving and mood (craving assessment 3) and rate the e-cig using the
e-cig rating form. (eye-tracking task, including time to calibrate participants
eye, takes approximately 30 minutes) Then, participants will be moved into an
adjoining research room to complete what researchers are presenting as the motor
coordination task. This room is equipped with a comfortable recliner, TV, and
computer. Participants will be permitted to use their e-cig throughout the session
and will sample 5 beers (two are non-alcoholic). Participants will be asked to
self-report e-cig and alcohol craving and mood (craving assessment 4) and will then
be asked to complete a beer taste test (15 minutes for technician to set up beer
taste testing). The researcher will ask participants to continue to use their e-cig
as they would like throughout the taste test portion of the study. Participants
will be given 5 minutes prior to the beginning of the taste test to take 10 puffs
of their e-cig. Prior to beginning the taste test, participants will be reminded
that they will be dismissed from the study as scheduled and will have to stay for
the entire allotted study time, regardless of the amount of beer they consume
during the session. They will be given their beer-rating sheet and the researcher
will leave the room. Participants will be unaware that the researcher, trained in
session coding, will record the timing of e-cig puffs and sips of beer temporally,
through the use of the lab's one-way mirror.

Three beers with similar per volume alcohol content (Bud Light- 4.2% alcohol,
Miller Light-4.2% alcohol, Sam Adams Light-4.1% alcohol) will be provided. In order
to limit overall breath alcohol content of participants, Buckler's and O'Doul's
(both 0% alcohol) will also be provided. All drinks will be measured (in mL) by a
highly trained research assistant over the age of 21, and poured into separate cold
glasses. Beers will be placed on a table in front of where the participant will be
seated. In session 2, participants will be told researchers have selected their
previously rated favorite beer from session 1, along with new beers to rate.
Participants will be told to drink as much or as little of each beer as they like
and rate each beer on various qualities (e.g., taste, aroma, drinkability), but to
at least drink enough to rate each beer. Participants will be instructed that they
have approximately 60 minutes to complete the taste test.

After approximately 60 minutes, participants will have their BrAC measured (rinsing
with water prior to measurement), will report their alcohol and e-cig craving and
mood (craving assessment 5), and beers will be removed and measured (in mL).
Participants will complete a field sobriety test which researchers are presenting
as the motor coordination portion of the study. The participant's e-cig will be
weighed at this time to calculate overall nicotine dose administered during the ad
libitum session. Participants will be then be given access to snacks and movies and
asked to remain in the room until the end of the session and their BrAC is at or
below 0.02 and they show no detectable signs of intoxication on a field sobriety
test. Preliminary data suggest that, on average, college students consume
approximately half of the beer presented during ad lib drinking sessions. The
maximum BrAC expected during the ad libitum session is approximately .05 (driving
limit is .08 in the State of Indiana), with the maximum expected time of
approximately 2.5 hours to return participants' BrAC to 0.02 or less (see Table 3);
therefore, this is the time set across all participants. On average, participants
reach a BrAC of zero after approximately 1-1.5 hours. Therefore, the researchers
have set 1.25 hours as the time across all participants for the sobering up period;
however, if participants BrAC is above .02 at the 1.25 hour mark, they will stay in
the lab until they reach a BrAC of .02 or less.

Participants will be allowed to use their e-cig ad libitum during the 60-minute
session. Each participant will use as much or as little of the e-cig as they would
like. Estimates of nicotine exposure to 18-24% nicotine content e-cigs results in
.08mg/ml blood concentration over a one-hour period. Therefore, researchers expect
the exposure to be in the range of ¼ - 1/5 of this amount, or, approximately
0.016-0.02 mg/ml blood concentration. Toxic levels are approximately 200 mg/ml.

After this time has passed, participants will have their sobriety verified, and
will complete a manipulation check, in which they will be asked what they believed
to be the goals of the study, in order to assess for the believability of the beer
taste test. Then, as the session is ending, the study technician will make
conversation with the participant and will ask about what the participant did the
day of the session and what s/he is doing the day after the session. This is to
assess the effect of outside obligations on drinking behaviors. Participants who
note significant obligations or guess study hypotheses will have their data
excluded from analysis. Finally, participants will be compensated and then
dismissed.

2. In the no e-cig cue condition participants will sit for five minutes with a pencil
in their hand (similar to an e-cig in size and weight). All participants will
report e-cig and alcohol craving and mood (craving assessment 2) and complete the
dot probe task and the beer taste test, as described above in the e-cig cue
condition, except that no e-cig will be used throughout any portion of the study.

4. In session 2, participants will complete: breathalyzer, urine drug and pregnancy screen,
and either the e-cig cue condition or no e-cig cue condition, as described above.

5. At the end of session 2, participants will be debriefed. Research Assistants will be
thoroughly trained in handling any concerns participants may have following the test,
and participants will be given study information, including contact information for the
study sponsor, department, and university, as well as counseling services, if
appropriate.

In order to diminish the possibility that subjects will leave before official release, the
following steps will be taken: 1. Subject keys will be taken upon arrival to the session and
returned upon dismissal; 2. Subjects who insist on leaving before these conditions are met
will not be paid or rescheduled for further testing and their keys will not be returned if
their BrAC is above 0.02, 3. Subjects will be kept in a private room that only exits into
laboratory space and will be monitored appropriately by staff to avoid early self-dismissal;
4. Should a subject become belligerent or insist upon leaving when not safe, the PI will be
notified to assist to ensure subject safety.

Inclusion Criteria:

- Healthy men and women, 21 years of age or older

- Regular alcohol use (reports at least one drink weekly - acceptability of average
alcohol consumption will be made on a case by case basis by the PI)

- No current/prior severe alcohol or substance use disorder (endorsement of > 6
Alcohol/Drug Use history items on the phone screen)

- Current e-cigarette use (reports at least weekly use - acceptability of average e-cig
use will be made on a case by case basis by the PI)

- Able to understand questionnaires/procedures in English

- Reports drinking beer (Yes/No on phone screen)

Exclusion Criteria:

- Pregnant/breast-feeding women (assessed via phone screen and urine screen)

- Unstable or significant medical disorders that may influence study outcome or
participant safety as determined by Melissa Cyders (e.g., liver condition)

- Current/prior severe alcohol use disorder, current/prior severe substance use disorder
(endorsement of > 6 Alcohol/Drug Use history items on the phone screen)

- Positive urine drug screen for amphetamines/meth-amphetamines, barbiturates,
benzodiazapines, cocaine, opiates, or PCP [1-phenylcyclohexyl]

- Current mental health problems, such as depression, anxiety, post-traumatic stress
disorder, bipolar disorder, or schizophrenia (self-reported at phone screen)

- Positive BrAC reading at the start of any study visit

- Court-mandated order to not drink alcohol

- Any condition that, in the judgment of the PI, could place the participant at risk or
affect data validity