Treatment of Cytomegalovirus (CMV) Infections With Viral-Specific T Cells

Inclusion Criteria:

1. Adult or pediatric patient suffering from CMV reactivation/infections following HSCT
or due to other immunocompromised states (e.g.; primary immunodeficiency, cytotoxic
therapy).

- CMV reactivation/viremia defined as positive (>500 copies/ml) CMV qPCR and/or

- Presence of symptoms secondary to CMV infection or evidence of invasive CMV
infection (e.g. pneumonitis, colitis) AND

- Patients must have ONE OF THE FOLLOWING CRITERIA:

- Absence of an improvement of viral load after ≥ 14 days of antiviral therapy
with ganciclovir, valganciclovir or foscarnet (decrease by at least 1 log,
i.e. 10-fold), or

- New, persistent and/or worsening CMV-related symptoms, signs and/or markers
of end organ compromise while on antiviral therapy with ganciclovir,
valganciclovir or foscarnet, or

- Have contraindications or experience adverse effects of antiviral therapy
with ganciclovir, valganciclovir or foscarnet, or

- Known resistance to ganciclovir and/or foscarnet based on molecular testing.

2. Recipients of an allogeneic HSCT must be 28 days after stem cell infusion at the time
of T-cell transfer.

3. Written informed consent given by patient or legal representative.

4. Minimum patient age 1 month.

5. Minimum weight 7 lbs.

6. Female patients of childbearing age with negative pregnancy tests.

7. Patient Karnofsky/Lansky Performance Status >30%.

8. Donor eligible based on FACT infectious screening requirements.

Exclusion Criteria:

1. Patient with acute GVHD > grade 2 or active moderate or severe chronic GVHD at time of
T-cell transfer

2. Patient receiving steroids (>0.5 mg/kg body weight (BW) prednisone equivalent) at the
time of T-cell transfer

3. Patient received allogeneic HSCT less than 28 days prior to T-cell transfer

4. Patient treated with donor lymphocyte infusion (DLI) within 28 days prior to T-cell
transfer

5. Patient treated with Thymoglobulin (ATG), Alemtuzumab or T-cell immunosuppressive
monoclonal antibodies within 28 days.

6. Patient with organ dysfunction or failure as determined by Karnofsky (patients >16
years) or Lansky (patients ≤16 years) score ≤30% (Appendix 5)

7. Patients with CMV retinitis

8. Concomitant enrollment in another clinical trial with endpoints interfering with this
study

9. Any medical condition which could compromise participation in the study according to
the investigator's assessment

10. Known HIV infection

11. Female patient who is pregnant or breast-feeding, or adult of reproductive potential
not willing to use an effective method of birth control during study treatment. Note:
Women of childbearing potential must have a negative serum pregnancy test at study
entry.

12. Patients unwilling or unable to comply with the protocol or unable to give informed
consent.

Donor Eligibility:

The original donor will be the first choice as source of T cells. If the original donor is
not available for donation (such as NMDP donor, cord blood unit, or related donor not
available) of peripheral mononuclear cells or does not meet all donor eligibility criteria
(including donor selection criteria based on University of Wisconsin - Madison Standard
Operating Procedures for the selection of allogeneic donors), alternative related donors
will be selected, with preference for those who have full HLA matching in 6/6 loci over
those with partial HLA matching (≥ 3/6 HLA loci). See Appendix 1 and 2 for patient and
donor screening procedures.

1. All donors must be ≥ 18 years old, available, CMV IgG positive, eligible and capable
of undergoing a single standard 2 blood volume leukapheresis. If original HSCT donor
is not available, CMV IgG negative or ineligible, a CMV IgG positive fully matched or
haploidentical family donor will be used.

2. Related donors must be at least partially HLA compatible, matching with recipient in
at least 3/6 HLA loci (HLA-A, HLA-B and HLA-DRB1 will be considered for this).

3. Donors must be CMV IgG seropositive.

4. Donors must show CMV T-cell activation after incubation with MACS GMP PepTivator
Peptide Pools of CMV pp65 before undergoing leukapheresis.

5. Donor must meet the criteria for donor selection defined in the Standard Operating
Procedures of the University of Wisconsin Hospitals and Clinics Stem Cell Transplant
Program and in FACT standards.