Biofilm Modified Macrophage Phenotype and Function in Diabetic Wound Healing
| Status: | Not yet recruiting |
|---|---|
| Conditions: | Infectious Disease, Hospital, Diabetes |
| Therapuetic Areas: | Endocrinology, Immunology / Infectious Diseases, Other |
| Healthy: | No |
| Age Range: | 18 - 80 |
| Updated: | 3/17/2019 |
| Start Date: | March 2019 |
| End Date: | February 2021 |
| Contact: | Jennifer Mohnacky, RDN |
| Email: | jmohnack@iu.edu |
| Phone: | 317-278-2715 |
The aim is to study the microenvironment of chronic biofilm infection in diabetic wounds
which shows deregulated immune cells responses and biofilm interaction resulting in
delayed/impaired/non wound healing. We seek to understand immune evasion strategy of biofilms
and study mechanisms which biofilm modify macrophages phenotype and functions in such wounds
which shows deregulated immune cells responses and biofilm interaction resulting in
delayed/impaired/non wound healing. We seek to understand immune evasion strategy of biofilms
and study mechanisms which biofilm modify macrophages phenotype and functions in such wounds
Specific Objective:
1. Determine if functionally active macrophage isolated from biofilm-infected diabetic
wounds show extra cellular matrix degrading phenotype causing wound collagen degradation
2. Understand by investigating whether increased presence of ECM degrading wound macrophage
phenotypes in biofilm infected human diabetic wounds are associated with reduced wound
collagen levels and increased wound recurrence End goal will be to understand the
mechanism in which biofilm modulates the wound macrophage phenotype and function
1. Determine if functionally active macrophage isolated from biofilm-infected diabetic
wounds show extra cellular matrix degrading phenotype causing wound collagen degradation
2. Understand by investigating whether increased presence of ECM degrading wound macrophage
phenotypes in biofilm infected human diabetic wounds are associated with reduced wound
collagen levels and increased wound recurrence End goal will be to understand the
mechanism in which biofilm modulates the wound macrophage phenotype and function
Inclusion Criteria:
1. Age 18 and above years old
2. Willing and able to provide informed consent
3. Willing and able to comply with protocol instructions, including biopsies and study
visits
4. Diabetics with an open wound
5. Receiving Negative Wound Pressure Therapy (NPWT)
Exclusion Criteria:
1. Inadequate arterial supply, as evidenced by any of the following (for wounds below the
knee):
1. TcOM < 30mmHg
2. ABI < 0.7
3. TBI < 0.6
2. Women who are pregnant
We found this trial at
1
site
Indianapolis, Indiana 46202
Principal Investigator: Sashwati Roy, Ph.D
Phone: 317-278-2715
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