Use of Multiphoton Microscopy in the Diagnosis of Cancer

Multiphoton microscopy (MPM) has been shown to be able to image tissue at a cellular level.
Our project will initially evaluated the ability of MPM imaging to distinguish normal
bladder urothelium from atypical and malignant urothelium in the ex vivo setting. After
development of sufficient criteria, we plan to develop an endoscopic bladder probe that will
provide a non-invasive means to image the interior of the bladder at the cellular level,
which would provide direct evidence of the presence of tumor without a biopsy. After
exhibiting usefulness of MPM imaging for bladder cancer, we will look at other organs
beginning with the colon.