Novel Approach to Assess Metabolic Flexibility in a Respiratory Chamber

Inclusion Criteria:

- Eligibility criteria include:

- Healthy male or female

- Ages 18-40 y

- BMI between 20 kg/m2 and 30 kg/m2 (±0.5 kg/m2 will be accepted)

- Are willing to consume pre-prepared meals

- Are willing to maintain current physical activity, sleep schedule, and dietary
habits during the study.

- Medically cleared for participation in the study by Medical Investigator

Exclusion Criteria:

- Participants are ineligible to participate (or will be excluded from participating in
this study) if they meet any of the following criteria:

- Unstable weight in the last 3 months [gain or loss >7 lb (or 3.2 kg)]

- Currently working shift work

- Smoking or use of tobacco products within the last 3 months

- Ammenorrhea (or absence of regular monthly cycles)

- History of clinically diagnosed diabetes or a fasting blood glucose >126 mg/dL

- Average screening blood pressure >140/90 mmHg

- Previous bariatric surgery (or other surgeries) for obesity or weight loss

- Use of medications affecting metabolism or sleep

- History of neurological disease

- History of cardiovascular disease (or other chronic diseases)

- Pregnant, planning to become pregnant, or breastfeeding

- Adherence to special restrained diets (e.g., low-CHO, low-fat, or
vegetarian/vegan diets) over the last 3 months.