Association Between Lactase Deficiency, and the Small Intestinal Microbiome in Adults.

The study involves 3 visits and will include 2 types of subjects - those who have the the
diagnosis of lactose intolerance (subjects) and those who do not have lactose intolerance
(controls).

Visit 1:

In total, it is estimated that the research participation will add about 1 hour to your
planned procedure visit. At Visit 1 following will be done

- Subjects will be asked to read and sign the informed consent after all the questions
about the study have been answered.

- General medical history will be taken including demographics, symptoms and history of
illness, and medication review.

- Vital signs (blood pressure, temperature, respiration, height, and weight) will be
recorded.

- Subjects will be asked to complete 2 questionnaires

- Upper endoscopy will be done as part of routine clinical care

- At the time of the endoscopy 4 extra biopsies (small piece of tissue) from the small
intestines will be taken. The biopsies will be used for research to detect the bacteria
and to measure the lactase enzyme.

- Small sample of blood (10 mL) (2 teaspoons) will be taken for storage for possible
future testing. This will be a one time draw only.

- The research team may contact the subjects to arrange a Visit 2 if they are interested
in proceeding with the next step in this research study.

Visit 2:

Visit 2 will take approximately 6 hours to complete. If the subject returns for Visit 2, they
will do the following

- They will return to the research office at Indiana University to participate in hydrogen
breath test (test that uses the measurement of hydrogen in the breath to diagnose
several conditions) to see how the body reacts to lactose. This involves drinking milk,
then blowing into a bag so we can collect breath samples. Subjects will be asked to
report any symptoms they might have during this time. This test will take approximately
6 hours. They will be instructed to eat a low-fiber meal the night before the test, and
asked to fast for the 12 hours before the test. They can drink water during this time.

- They will be also be asked to provide a stool sample for future analysis. Instructions
for collection, storage and transportation of stool will be provided. They will be
provided a kit for stool collection, storage and transportation. They will be asked to
collect stool at home over the course of 2 days and store as per instructions. Stool
will need to be refrigerated and brought back to the office on ice.

Visit 3:

Visit 3 will take approximately less than one hour to complete. For those who are interested
in returning for Visit 3,

- Subjects will be asked to return to the clinic with the stool samples.

- They will complete the study at this time.

Participants who complete Aim 1 and meet further eligibility criteria for Aim 2 may be
contacted by research staff to return for Visit 2 (breath test). Those who are agreeable to
participating and are scheduled for Visit 2 will be instructed to consume a low-fiber meal
and fast for 12 hours prior to their breath test. We will contact the participant 24 to 48
hours prior to the test day as a reminder to consume a low fiber meal and fast for 12 hours.

Patient ages 18-75 presenting for upper gastrointestinal endoscopy for any indication
except those outlined in the exclusion criteria below at the IU GI Endoscopy Sites
including: IU North Hospital, Glen Lehman Endoscopy Suite, Springmill/Beltway Surgery
Center, IU Saxony, and Eskenazi Hospital will be eligible for participation. Participants
should be willing and able to sign informed consent.

Inclusion Criteria for Controls (Aim 1): Adult patients ages 18-75 years meeting basic
eligibility criteria as listed above will be considered for inclusion. Participants should
report no current or recent history of intolerance to and avoidance of dairy nor use of
treatments and products for dairy intolerance.

Inclusion Criteria for Patients with Self-Reported Lactose Intolerance (Aim 1): Adult
patients ages 18-75 years meeting basic eligibility criteria as listed above will be
considered for inclusion. Participants should report current or recent history of
intolerance to and avoidance of dairy.

Inclusion Criteria for Controls (Aim 2): All controls eligible for and completing Aim 1
will be considered eligible for inclusion. Participants should not meet any of the Aim 2
exclusion criteria.

Inclusion Criteria for Patients with Self-Reported Lactose Intolerance (Aim 1): All
patients with self-reported lactose intolerance eligible for and completing Aim 1 will be
considered eligible for inclusion. Participants should not meet any of the Aim 2 exclusion
criteria.

Exclusion criteria (Aim 1):

1. Patients with known bleeding diathesis given the need for small intestinal mucosal
biopsies.

2. Patients with known history of eosinophilic esophagitis, inflammatory bowel disease,
chronic pancreatitis or pancreatic insufficiency, celiac disease, peptic duodenitis

3. Antibiotic-use within the last 30 days

4. Major abdominal surgery except appendectomy or cholecystectomy > 6 months prior to
enrollment

5. Prior radiation therapy of the abdomen

6. Use of tobacco products within the last 3 months

7. Allergy to milk

8. Currently pregnant or breast-feeding

9. Patients taking anti-coagulant drugs

Exclusion criteria (Aim 2):

1. Meeting any of the exclusion criteria as listed for Aim 1

2. Known diagnosis of disorders associated with abnormal GI motility (gastroparesis,
amyloidosis, neuromuscular disease including Parkinson's disease, collagen vascular
disease, chronic malnutrition, and uncontrolled thyroid disease)

3. History of small intestinal bacterial overgrowth

4. Active peptic ulcer disease noted at the time of endoscopy

5. Recent colonoscopy bowel preparation or colonic enema in the preceding 4 weeks

6. Chronic immunodeficiency or chronic infectious illness

7. Currently undergoing chemotherapy

8. Patients taking anti-coagulant drugs