e-Cigarette - DNA Adducts

In this observational study, e-cigarette users, smokers, and non-users of e-cigarette or
tobacco products (up to 134 per group) will be recruited from the Minneapolis-St. Paul area.
This research will be conducted at the Tobacco Research Programs at the University of
Minnesota. Subjects are screened for eligibility over the phone. If eligible after the phone
screen, participants will be invited to attend orientation screening visit in the clinic
where consent will be obtained and subjects will complete forms about their tobacco and
e-cigarette use and medical history. Subjects who eligible will be asked to return for six
visits over the course of six months.

At these appointments, subjects will provide biological specimen samples (buccal cells, urine
and blood). Saliva will be collected before and after a smoking or vaping session. Non-users
will only have 1 saliva collection.

Subjects will be asked for information pertaining to and e-cigarette and tobacco use and
overall health.

Inclusion Criteria:

- Good physical health with no unstable medical conditions; Able to provide written
informed consent; Stable and good mental health;

Meet criteria for 1 of three groups:

1. Regular smokers confirmed by CO;

2. E-cigarette users (exclusive e-cigarette use for 3 months); Non-Tobacco Users

3. Non-smokers

Exclusion Criteria:

- Unstable medical condition Not willing to attend visits